Cell Culture and Tissue Engineering

Differentiation of PC12 cells in three-dimensional collagen sponges with micropatterned nerve growth factor

  1. Hwan Hee Oh1,2,
  2. Hongxu Lu3,
  3. Naoki Kawazoe3,
  4. Guoping Chen4,5,*

Article first published online: 16 FEB 2012

DOI: 10.1002/btpr.1520

Issue

Biotechnology Progress

Biotechnology Progress

Volume 28, Issue 3, pages 773–779, May/June 2012

Additional Information

How to Cite

Oh, H. H., Lu, H., Kawazoe, N. and Chen, G. (2012), Differentiation of PC12 cells in three-dimensional collagen sponges with micropatterned nerve growth factor. Biotechnol Progress, 28: 773–779. doi: 10.1002/btpr.1520

Author Information

  1. 1

    Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Tsukuba, Ibaraki 305-0044, Japan

  2. 2

    Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan

  3. 3

    Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

  4. 4

    Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

  5. 5

    Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, Japan

*Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

Publication History

  1. Issue published online: 9 JUN 2012
  2. Article first published online: 16 FEB 2012
  3. Accepted manuscript online: 17 JAN 2012 04:19PM EST
  4. Manuscript Revised: 22 DEC 2011
  5. Manuscript Received: 12 OCT 2011

Funded by

  • World Premier International Research Center Initiative (WPI)
  • Ministry of Education, Culture, Sports, Science and Technology, Japan

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Keywords:

  • neuronal differentiation;
  • PC12 cells;
  • NGF;
  • collagen sponge;
  • micropattern

Abstract

Micropatterning of biological cues is important for the guided formation of neuronal outgrowth and neuronal differentiation. Nerve growth factor (NGF) was micropatterned in a three-dimensional collagen sponges by using micropatterned ice lines that were composed of collagen and NGF. The micropatterned ice lines were prepared by a dispersing machine. PC12 cells were cultured in the NGF-micropatterned collagen sponges and showed micropatterned neurite outgrowth. The neurite outgrowth followed the micropattern of NGF with more neurite outgrowth in the collagen/NGF lines than in the regions between the collagen/NGF lines. The micropattern of the NGF and the neurite network of the PC12 cells can be manipulated by controlling the micropattern of the NGF. The three-dimensional porous scaffolds prepared by this method will have a potential application for the regeneration and repair of the nervous system. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012

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