Immobilization of Escherichia coli containing ω-transaminase activity in LentiKats®

Authors

  • Max Cárdenas-Fernández,

    1. Dept. of Chemical Engineering, Applied Biocatalysis Unit associated to IQAC (UAB-CSIC), School of Engineering, Universitat Autònoma de Barcelona, 08193-Bellaterra (Cerdanyola del Vallès), Catalunya, Spain
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  • Watson Neto,

    1. Dept. of Chemical and Biochemical Engineering, Center for Process Engineering and Technology, Technical University of Denmark, DK-2800 Lyngby, Denmark
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  • Carmen López,

    1. Dept. of Chemical Engineering, Applied Biocatalysis Unit associated to IQAC (UAB-CSIC), School of Engineering, Universitat Autònoma de Barcelona, 08193-Bellaterra (Cerdanyola del Vallès), Catalunya, Spain
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  • Gregorio Álvaro,

    1. Dept. of Chemical Engineering, Applied Biocatalysis Unit associated to IQAC (UAB-CSIC), School of Engineering, Universitat Autònoma de Barcelona, 08193-Bellaterra (Cerdanyola del Vallès), Catalunya, Spain
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  • Pär Tufvesson,

    Corresponding author
    1. Dept. of Chemical and Biochemical Engineering, Center for Process Engineering and Technology, Technical University of Denmark, DK-2800 Lyngby, Denmark
    • Center for Process Engineering and Technology, Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Lyngby, Denmark
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  • John M. Woodley

    1. Dept. of Chemical and Biochemical Engineering, Center for Process Engineering and Technology, Technical University of Denmark, DK-2800 Lyngby, Denmark
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Abstract

Whole Escherichia coli cells overexpressing ω-transaminase (ω-TA) and immobilized cells entrapped in LentiKats® were used as biocatalysts in the asymmetric synthesis of the aromatic chiral amines 1-phenylethylamine (PEA) and 3-amino-1-phenylbutane (APB). Whole cells were permeabilized with different concentrations of cetrimonium bromide (CTAB) and ethanol; the best results were obtained with CTAB 0.1% which resulted in an increase in reaction rate by 40% compared to the whole cells. The synthesis of PEA was carried out using isopropyl amine (IPA) and L-alanine (Ala) as amino donors. Using whole cell biocatalysis, the reaction with IPA was one order of magnitude faster than with Ala. No reaction was detected when permeabilized E. coli cells containing ω-TA were employed using Ala as the amino donor. Additionally, the synthesis of APB from 4-phenyl-2-butanone and IPA was studied. Whole and permeabilized cells containing ω-TA and their immobilized LentiKats® counterparts showed similar initial reactions rates and yields in the reaction systems, indicating 100% of immobilization efficiency (observed activity/activity immobilized) and absence of diffusional limitations (due to the immobilization). Immobilization of whole and permeabilized cells containing ω-TA in LentiKats® allowed improved stability as the biocatalyst was shown to be efficiently reused for five reaction cycles, retaining around 80% of original activity. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012

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