Bioseparations and Downstream Processing

Human collagen isolated from adipose tissue

  1. Beob Soo Kim1,
  2. Ji Suk Choi1,
  3. Jae Dong Kim1,
  4. Hwa In Yoon1,
  5. Young Chan Choi1,
  6. Yong Woo Cho2,*

Article first published online: 8 JUN 2012

DOI: 10.1002/btpr.1555

Issue

Biotechnology Progress

Biotechnology Progress

Volume 28, Issue 4, pages 973–980, July/August 2012

Additional Information

How to Cite

Kim, B. S., Choi, J. S., Kim, J. D., Yoon, H. I., Choi, Y. C. and Cho, Y. W. (2012), Human collagen isolated from adipose tissue. Biotechnol Progress, 28: 973–980. doi: 10.1002/btpr.1555

Author Information

  1. 1

    Dept. of Bionanotechnology, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea

  2. 2

    Dept. of Chemical Engineering, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea

*Dept. of Chemical Engineering, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea

Publication History

  1. Issue published online: 7 AUG 2012
  2. Article first published online: 8 JUN 2012
  3. Accepted manuscript online: 2 MAY 2012 03:33AM EST
  4. Manuscript Revised: 9 APR 2012
  5. Manuscript Received: 4 NOV 2011

Funded by

  • Basic Science Research Program. Grant Number: 2009-0075546
  • Bio & Medical Technology Development Program. Grant Number: 2011-0019774
  • National Research Foundation of Korea (NRF)
  • Korean government (MEST)
  • Seoul R&BD Program. Grant Number: SS110011)

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Keywords:

  • collagen;
  • adipose tissue;
  • liposuction;
  • extracellular matrix;
  • extraction

Abstract

Collagen, the most abundant protein in vertebrates, is a useful biomaterial in pharmaceutical and medical industries. So far, most collagen has been extracted from animals and cadavers. Herein, we suggest human adipose tissue, which is routinely abandoned after liposuction, as a plentiful source of human collagen. In this study, human collagen was obtained from adipose tissue through two successive major steps: (i) extraction of the extracellular matrix (ECM) by pulverization, centrifugation, alkaline, and alcohol treatment; (ii) isolation of collagen from ECM by pepsin treatment in dilute acetic acid. The purified human adipose-derived collagen was characterized by Fourier transform infrared spectroscopy, polyacrylamide gel electrophoresis, amino acid analysis, and circular dichroism spectroscopy. The extracted collagen showed a typical triple helix structure, good thermal stability due to abundant imino acids, and high solubility at acidic pH. The collagen greatly facilitated the adhesion and proliferation of human adipose-derived stem cells and normal human dermal fibroblasts on polystyrene plates. These results suggest that human adipose tissue obtained by liposuction can provide human collagen for use in cosmetics, pharmaceutics, and medicine. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 28: 973–980, 2012

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