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Process Sensing and Control
In situ Raman spectroscopy for simultaneous monitoring of multiple process parameters in mammalian cell culture bioreactors
Article first published online: 20 JUL 2012
DOI: 10.1002/btpr.1590
Copyright © 2012 American Institute of Chemical Engineers (AIChE)
Additional Information
How to Cite
Whelan, J., Craven, S. and Glennon, B. (2012), In situ Raman spectroscopy for simultaneous monitoring of multiple process parameters in mammalian cell culture bioreactors. Biotechnol Progress, 28: 1355–1362. doi: 10.1002/btpr.1590
Publication History
- Issue published online: 10 OCT 2012
- Article first published online: 20 JUL 2012
- Accepted manuscript online: 28 JUN 2012 02:00AM EST
- Manuscript Revised: 19 JUN 2012
- Manuscript Received: 16 MAY 2012
Funded by
- Enterprise Ireland
- Abstract
- Article
- References
- Cited By
Keywords:
- Raman spectroscopy;
- bioprocess monitoring;
- CHO fed-batch;
- PLS models;
- process analytical technology
Abstract
In this study, the application of Raman spectroscopy to the simultaneous quantitative determination of glucose, glutamine, lactate, ammonia, glutamate, total cell density (TCD), and viable cell density (VCD) in a CHO fed-batch process was demonstrated in situ in 3 L and 15 L bioreactors. Spectral preprocessing and partial least squares (PLS) regression were used to correlate spectral data with off-line reference data. Separate PLS calibration models were developed for each analyte at the 3 L laboratory bioreactor scale before assessing its transferability to the same bioprocess conducted at the 15 L pilot scale. PLS calibration models were successfully developed for all analytes bar VCD and transferred to the 15 L scale. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012