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Bioseparations and Downstream Processing

Investigating the use of column inserts to achieve better chromatographic bed support

  1. Tian Lan1,
  2. Spyridon Gerontas1,
  3. Graeme R. Smith1,
  4. John Langdon1,
  5. John M. Ward2,
  6. Nigel J. Titchener-Hooker3,*

Article first published online: 27 JUL 2012

DOI: 10.1002/btpr.1597

Issue

Biotechnology Progress

Biotechnology Progress

Volume 28, Issue 5, pages 1285–1291, September/October 2012

Additional Information

How to Cite

Lan, T., Gerontas, S., Smith, G. R., Langdon, J., Ward, J. M. and Titchener-Hooker, N. J. (2012), Investigating the use of column inserts to achieve better chromatographic bed support. Biotechnol Progress, 28: 1285–1291. doi: 10.1002/btpr.1597

Author Information

  1. 1

    The Advanced Centre for Biochemical Engineering, Dept. of Biochemical Engineering, University College London, London WC1E 7JE, U.K.

  2. 2

    Research Department of Structural and Molecular Biology, University College London, London WC1E 6BT, U.K.

  3. 3

    The Advanced Centre for Biochemical Engineering, Dept. of Biochemical Engineering, University College London, London WC1E 7JE, U.K.

*The Advanced Centre for Biochemical Engineering, Dept. of Biochemical Engineering, University College London, London WC1E 7JE, U.K.

Publication History

  1. Issue published online: 10 OCT 2012
  2. Article first published online: 27 JUL 2012
  3. Accepted manuscript online: 2 JUL 2012 11:44PM EST
  4. Manuscript Revised: 27 JUN 2012
  5. Manuscript Received: 27 FEB 2012

Funded by

  • Engineering and Physical Sciences Research Council (EPSRC)
  • EPSRC Centre for Innovative Manufacture in Emergent Macromolecular Therapies

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Keywords:

  • column insert;
  • critical velocity;
  • wall support;
  • compression;
  • column efficiency

Abstract

Chromatography plays an important role in the downstream processing of proteins. Over the past years, there has been a steady move toward the adoption of more rigid, porous particles to combine ease of manufacture with increased levels of productivity. The latter is still constrained by the onset of compression where the level of wall support becomes incapable of withstanding flow-induced particle drag. In this study, we investigate how, by the installation of cylindrical column inserts, it is possible to enhance the level of wall support. Experiments were conducted to examine the effect of the position of the insert in the column, and also of the insert dimensions on the critical velocity at which the onset of compression occurs. It was found that when installed at the bottom of the column, inserts can provide up to a 20% increase in critical velocity without significantly affecting column hydrodynamics, as measured by the level of axial dispersion. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012

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