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Biocatalysts and Bioreactor Design
Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus
Article first published online: 28 AUG 2012
DOI: 10.1002/btpr.1602
Copyright © 2012 American Institute of Chemical Engineers (AIChE)
Additional Information
How to Cite
De Benedetti, E. C., Rivero, C. W., Britos, C. N., Lozano, M. E. and Trelles, J. A. (2012), Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus. Biotechnol Progress, 28: 1251–1256. doi: 10.1002/btpr.1602
Publication History
- Issue published online: 10 OCT 2012
- Article first published online: 28 AUG 2012
- Accepted manuscript online: 27 JUL 2012 05:18AM EST
- Manuscript Revised: 13 JUL 2012
- Manuscript Received: 23 MAY 2012
Funded by
- Ministerio de Ciencia, Tecnología e Innovación Productiva de la Nación Argentina and Universidad Nacional de Quilmes
- Abstract
- Article
- References
- Cited By
Keywords:
- thermophilic microorganisms;
- nucleoside phosphorylase;
- whole cell immobilization;
- prodrug precursors;
- transglycosylation
Abstract
An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2′-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2′-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012