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Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus

Authors

  • Eliana C. De Benedetti,

    1. Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal (B1868BXD), Argentina
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  • Cintia W. Rivero,

    1. Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal (B1868BXD), Argentina
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  • Claudia N. Britos,

    1. Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal (B1868BXD), Argentina
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  • Mario E. Lozano,

    1. Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal (B1868BXD), Argentina
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  • Jorge A. Trelles

    Corresponding author
    1. Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal (B1868BXD), Argentina
    • Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal (B1868BXD), Argentina
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Abstract

An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2′-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2′-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012

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