Cell Culture and Tissue Engineering

Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields

  1. Hyunjin Cho1,
  2. Young-Kwon Seo2,
  3. Hee-Hoon Yoon1,
  4. Soo-Chan Kim3,
  5. Sung-Min Kim2,
  6. Kye-Yong Song4,
  7. Jung-Keug Park5,*

Article first published online: 18 SEP 2012

DOI: 10.1002/btpr.1607

Issue

Biotechnology Progress

Biotechnology Progress

Volume 28, Issue 5, pages 1329–1335, September/October 2012

Additional Information

How to Cite

Cho, H., Seo, Y.-K., Yoon, H.-H., Kim, S.-C., Kim, S.-M., Song, K.-Y. and Park, J.-K. (2012), Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields. Biotechnol Progress, 28: 1329–1335. doi: 10.1002/btpr.1607

Author Information

  1. 1

    Dongguk University Research Institute of Biotechnology, Dongguk University, Seoul 100-715, Korea

  2. 2

    Dept. of Medical Biotechnology, Dongguk University, Seoul 100-715, Korea

  3. 3

    Graduate School of Bio and Information Technology, Hankyong National University, Anseong-si, Kyonggi-do 456-749, Korea

  4. 4

    Dept. of Pathology, Chungang University College of Medicine, 221 Heukseok dong, Seoul 156-756, Republic of Korea

  5. 5

    Dept. of Medical Biotechnology, Dongguk University Research Institute of Biotechnology, Dongguk University, Seoul 100-715, Korea

*Dongguk University Research Institute of Biotechnology, Dongguk University, Seoul 100-715, Korea

Publication History

  1. Issue published online: 10 OCT 2012
  2. Article first published online: 18 SEP 2012
  3. Accepted manuscript online: 31 JUL 2012 01:18AM EST
  4. Manuscript Revised: 10 JUL 2012
  5. Manuscript Received: 22 FEB 2012

Funded by

  • National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology. Grant Number: 2009-0082941

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (346K)

Keywords:

  • bone marrow mesenchymal stem cells;
  • extremely low frequency electromagnetic field;
  • neural cells

Abstract

Adult stem cells are considered multipotent. Especially, human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have the potential to differentiate into nerve type cells. Electromagnetic fields (EMFs) are widely distributed in the environment, and recently there have been many reports on the biological effects of EMFs. hBM-MSCs are weak and sensitive pluripotent stem cells, therefore extremely low frequency-electromagnetic fields (ELF-EMFs) could be affect the changes of biological functions within the cells. In our experiments, ELF-EMFs inhibited the growth of hBM-MSCs in 12 days exposure. Their gene level was changed and expression of the neural stem cell marker like nestin was decreased but the neural cell markers like MAP2, NEUROD1, NF-L, and Tau were induced. In immunofluorescence study, we confirmed the expression of each protein of neural cells. And also both oligodendrocyte and astrocyte related proteins like O4 and GFAP were expressed by ELF-EMFs. We suggest that EMFs can induce neural differentiation in BM-MSCs without any chemicals or differentiation factors. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012

View Full Article (HTML) Get PDF (346K)