Advertisement

In situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks

Authors

  • Ketaki Pradhan,

    1. Chemical Engineering and Process Development, CSIR-National Chemical Laboratory, Pune, Maharashtra, India 411008
    Search for more papers by this author
    • Ketaki Pradhan and Tejal Pant contributed equally to this work.

  • Tejal Pant,

    1. Chemical Engineering and Process Development, CSIR-National Chemical Laboratory, Pune, Maharashtra, India 411008
    Search for more papers by this author
    • Ketaki Pradhan and Tejal Pant contributed equally to this work.

  • Mugdha Gadgil

    Corresponding author
    1. Chemical Engineering and Process Development, CSIR-National Chemical Laboratory, Pune, Maharashtra, India 411008
    • Chemical Engineering and Process Development, CSIR-National Chemical Laboratory, Pune, Maharashtra, India 411008
    Search for more papers by this author

Abstract

pH in animal cell cultures decreases due to production of metabolites like lactate. pH control via measurement and base addition is not easily possible in small-scale culture formats like tissue-culture flasks and shake flasks. A hydrogel-based system is reported for in situ pH maintenance without pH measurement in such formats, and is demonstrated to maintain pH between 6.8 and 7.2 for a suspension CHO cell line in CD CHO medium and between 7.3 and 7.5 for adherent A549 cells in DMEM:F12 containing 10% FBS. This system for pH maintenance, along with our previous report of hydrogels for controlled nutrient delivery in shake flasks can allow shake flasks to better mimic bioreactor-based fed batch operation for initial screening during cell line and process development for recombinant protein production in mammalian cells. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012

Ancillary