Formulation and Engineering of Biomaterials

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Phosphatidylcholine enrichment with medium chain fatty acids by immobilized phospholipase A1-catalyzed acidolysis

  1. Angélica A. Ochoa1,2,
  2. Josafat A. Hernández-Becerra1,3,
  3. Adriana Cavazos-Garduño1,
  4. Hugo S. García1,*,
  5. Eduardo J. Vernon-Carter4

Article first published online: 21 NOV 2012

DOI: 10.1002/btpr.1648

Issue

Biotechnology Progress

Biotechnology Progress

Volume 29, Issue 1, pages 230–236, January/February 2013

Additional Information

How to Cite

Ochoa, A. A., Hernández-Becerra, J. A., Cavazos-Garduño, A., García, H. S. and Vernon-Carter, E. J. (2013), Phosphatidylcholine enrichment with medium chain fatty acids by immobilized phospholipase A1-catalyzed acidolysis. Biotechnol Progress, 29: 230–236. doi: 10.1002/btpr.1648

Author Information

  1. 1

    UNIDA, Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, Col. Formando Hogar, Veracruz, Ver. 91897, México

  2. 2

    Div. Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México

  3. 3

    Div. de Tecnología de Alimentos, Universidad Tecnológica de Tabasco, Villahermosa, Tabasco, México

  4. 4

    Dept. Ing. Procesos & Hidráulica, Universidad Autónoma Metropolitana Iztapalapa, Mexico City 09340, México

*UNIDA, Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, Col. Formando Hogar, Veracruz, Ver. 91897, México

Publication History

  1. Issue published online: 4 FEB 2013
  2. Article first published online: 21 NOV 2012
  3. Accepted manuscript online: 16 OCT 2012 07:44AM EST
  4. Manuscript Revised: 12 OCT 2012
  5. Manuscript Received: 7 JUN 2012

Funded by

  • National Council for Science and Technology of Mexico (CONACyT). Grant Number: 129334

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Keywords:

  • Acidolysis;
  • medium chain fatty acids;
  • phospholipase A1;
  • modified phosphatidylcholine

Abstract

Phospholipids are a biologically and industrially important class of compounds whose physical properties can be improved for diverse applications by substitution of medium-chain fatty acids for their native fatty acid chains. In this study, phosphatidylcholine (PC) was enriched with medium-chain fatty acids (MCFAs) by acidolysis with phospholipase A1 (PLA1) immobilized on Duolite A568. Response surface methodology was employed to evaluate the effects of the molar ratio of substrates (PC to free MCFAs), enzyme loading, and reaction temperature on the incorporation of free MCFAs into PC and on PC recovery. Enzyme loading and molar ratio of substrates contributed positively, but temperature negatively, to the incorporation of free MCFAs into PC. Increases in enzyme loading and the molar ratio of PC to free MCFAs led to increased incorporation of the latter into the former, but increased temperature had the opposite effect. By contrast, an increase in enzyme loading led to decreased PC recovery. Increased temperature had also a negative effect on PC recovery. Optimal conditions for maximum incorporation and PC recovery were molar ratio of PC to free MCFAs of 1:16, enzyme loading of 16%, and 50°C. Under these conditions, the incorporation of free MCFAs was 41% and the PC recovery was 53%. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2013

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