Phosphatidylcholine enrichment with medium chain fatty acids by immobilized phospholipase A1-catalyzed acidolysis

Authors

  • Angélica A. Ochoa,

    1. UNIDA, Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, Col. Formando Hogar, Veracruz, Ver. 91897, México
    2. Div. Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México
    Search for more papers by this author
  • Josafat A. Hernández-Becerra,

    1. UNIDA, Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, Col. Formando Hogar, Veracruz, Ver. 91897, México
    2. Div. de Tecnología de Alimentos, Universidad Tecnológica de Tabasco, Villahermosa, Tabasco, México
    Search for more papers by this author
  • Adriana Cavazos-Garduño,

    1. UNIDA, Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, Col. Formando Hogar, Veracruz, Ver. 91897, México
    Search for more papers by this author
  • Hugo S. García,

    Corresponding author
    1. UNIDA, Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, Col. Formando Hogar, Veracruz, Ver. 91897, México
    • UNIDA, Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, Col. Formando Hogar, Veracruz, Ver. 91897, México

    Search for more papers by this author
  • Eduardo J. Vernon-Carter

    1. Dept. Ing. Procesos & Hidráulica, Universidad Autónoma Metropolitana Iztapalapa, Mexico City 09340, México
    Search for more papers by this author

Abstract

Phospholipids are a biologically and industrially important class of compounds whose physical properties can be improved for diverse applications by substitution of medium-chain fatty acids for their native fatty acid chains. In this study, phosphatidylcholine (PC) was enriched with medium-chain fatty acids (MCFAs) by acidolysis with phospholipase A1 (PLA1) immobilized on Duolite A568. Response surface methodology was employed to evaluate the effects of the molar ratio of substrates (PC to free MCFAs), enzyme loading, and reaction temperature on the incorporation of free MCFAs into PC and on PC recovery. Enzyme loading and molar ratio of substrates contributed positively, but temperature negatively, to the incorporation of free MCFAs into PC. Increases in enzyme loading and the molar ratio of PC to free MCFAs led to increased incorporation of the latter into the former, but increased temperature had the opposite effect. By contrast, an increase in enzyme loading led to decreased PC recovery. Increased temperature had also a negative effect on PC recovery. Optimal conditions for maximum incorporation and PC recovery were molar ratio of PC to free MCFAs of 1:16, enzyme loading of 16%, and 50°C. Under these conditions, the incorporation of free MCFAs was 41% and the PC recovery was 53%. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2013

Ancillary