Bioseparations and Downstream Processing

You have free access to this content

Solubility properties and diffusional extraction behavior of natamycin from streptomyces gilvosporeus biomass

  1. Xianhai Zeng1,†,
  2. Michael K. Danquah2,
  3. Keju Jing1,
  4. Meng Wai Woo2,
  5. Xiao Dong Chen1,2,
  6. Youping Xie1,
  7. Yinghua Lu1,*

Article first published online: 4 DEC 2012

DOI: 10.1002/btpr.1659

Issue

Biotechnology Progress

Biotechnology Progress

Volume 29, Issue 1, pages 109–115, January/February 2013

Additional Information

How to Cite

Zeng, X., Danquah, M. K., Jing, K., Woo, M. W., Chen, X. D., Xie, Y. and Lu, Y. (2013), Solubility properties and diffusional extraction behavior of natamycin from streptomyces gilvosporeus biomass. Biotechnol Progress, 29: 109–115. doi: 10.1002/btpr.1659

Author Information

  1. 1

    Dept. of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

  2. 2

    Dept. of Chemical Engineering, Monash University, Clayton 3800 VIC, Australia

  1. †

    School of Energy Research, Xiamen University, Xiamen 361005, China

*Dept. of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Publication History

  1. Issue published online: 4 FEB 2013
  2. Article first published online: 4 DEC 2012
  3. Accepted manuscript online: 1 NOV 2012 08:21AM EST
  4. Manuscript Revised: 9 OCT 2012
  5. Manuscript Received: 25 APR 2012

Funded by

  • Cooperation of Industry, Education and Academy, Guangdong province and Ministry of Education, China. Grant Number: 2008B090500218
  • Project for Fujian Industrial Technology Development, Development and Reform Commission of Fujian Province, China. Grant Number: [2010] 299

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (439K)

Keywords:

  • natamycin;
  • Streptomyces gilvosporeus;
  • solubility;
  • extraction;
  • diffusion

Abstract

Natamycin is a type of polyene macrolide antibiotic and has been produced in submerged microbial cultures of some natural Streptomyces strains. Natamycin extraction from cellular biomass is greatly affected by the molecular and solubilization characteristics of the extraction solvent, and this is a major reason for the routine attainment of low volumetric titers, resulting from sparing natamycin solubility. In this work, a series of experiments were conducted to investigate the solubility of natamycin in some selected organic solvents in order to assess the influence on natamycin extraction yield. Natamycin showed the highest solubility in 75% aqueous methanol under the conditions of pH 2, 30°C and 1 atm. Furthermore, the extraction of natamycin using 75% aqueous methanol was performed and the highest extraction yield of 45.7% was obtained under pH 2. A mathematical model derived from Fick's law of the biomolecular diffusion process was developed to fit the experimental kinetic data of natamycin extraction. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2013

View Full Article (HTML) Get PDF (439K)