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Keywords:

  • antibody;
  • purification;
  • nonfucosylated;
  • FcγRIIIa;
  • FcγRIIIb;
  • glycoform

Post-translational modifications can dramatically impact protein activity, but identifying such structure:function relationships, as well as capitalizing on functionally enhanced variants, is a significant challenge. Here, affinity chromatography resins that contained immobilized FcγRIII receptors were used to enrich nonfucosylated antibodies 6- to 9-fold, offering what may be a tractable method for both the identification of post-translational modifications that affect function, as well as a means to enrich variants with enhanced activity. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:825–828, 2013