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Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa

Authors

  • Xiaoqin Wang,

    1. Lab. of Biotechnology, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, P.R. China
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  • Xuewu Zhang

    Corresponding author
    1. Lab. of Biotechnology, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, P.R. China
    • Correspondence concerning this article should be addressed to: X. Zhang at snow_dance@sina.com.

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Abstract

Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion exchange, and gel filtration chromatography, we separated a polypeptide that exhibited inhibitory activity on human liver cancer HepG2 cells, and named the polypeptide CPAP (C. pyrenoidosa antitumor polypeptide). Furthermore, the micro- and nanoencapsulation of CPAP were investigated by using two methods: complex coacervation and ionotropic gelation. The in vitro release tests revealed that CPAP was well preserved against gastric enzymatic degradation after micro/nanoencapsulation and the slowly controlled release in the intestine could be potentially achieved. These results suggest that CPAP may be a useful ingredient in food, nutraceutical, and pharmaceutical applications. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:681–687, 2013

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