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Correlation analysis of the taxane core functional group modification, enzyme expression, and metabolite accumulation profiles under methyl jasmonate treatment

Authors

  • Guang Hao Song,

    1. Inst. of Resource Biology and Biotechnology, Dept. of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
    2. Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
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    • Guang Hao Song, Chun Fang Zhao contributed equally to this work.

  • Chun Fang Zhao,

    Corresponding author
    1. Inst. of Resource Biology and Biotechnology, Dept. of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
    2. Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
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    • Guang Hao Song, Chun Fang Zhao contributed equally to this work.

  • Meng Zhang,

    1. Inst. of Resource Biology and Biotechnology, Dept. of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
    2. Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
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  • Chun Hua Fu,

    1. Inst. of Resource Biology and Biotechnology, Dept. of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
    2. Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
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  • Hua Zhang,

    1. Inst. of Resource Biology and Biotechnology, Dept. of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
    2. Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
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  • Long Jiang Yu

    Corresponding author
    1. Inst. of Resource Biology and Biotechnology, Dept. of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
    2. Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
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Abstract

Metabolomic and transcriptomic profiling data were obtained and integrated to elucidate the crucial network controls on taxol and its precursor biosynthesis during the taxane core functionalization within methyl jasmonate (MJ)-induced Taxus chinensis cells. Twelve metabolites were identified using liquid chromatography-electrospray ionization-mass spectrometry. These metabolites contain taxol (paclitaxel), baccatin III (B-III) and its analogs, a group structurally bearing multiple free hydroxyls (TAX), and another group of multiple acyl taxanes (MAT), including taxuyunnanine C (TC) and its analogs. The metabolomic profile showed a higher increase in TAX than in MAT. Particularly, the ratio of B-III and taxol to the total taxane content increased more significantly in TAX than in MAT. The MAT proportion did not significantly change, although they are predominant components in cell cultures compared with TAX. Quantitative real-time polymerase chain reaction (qRT-RCR) was used to determine the transcription level of 20 genes, among which 11 were reported responsible for taxol biosynthesis and 9 were obtained from our previous transcriptomic data. The total expression levels of hydroxylase after 24 h and 6 days were higher than those of acylase. The principal component analysis (PCA) results validated the metabolomic analysis data, indicating that hydroxylation was more crucial than acylation for controlling the flux toward TAX biosynthesis. Furthermore, the PCA contribution comparison showed that two undefined genes of OHX1 and ACX3 might have good potential in TAX upregulation and MAT downregulation. To the best of our knowledge, this study provides the first experimental evidence on the contribution of total hydroxylation to taxane biosynthesis. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 30:269–280, 2014

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