Process Sensing and Control

Improved particle counting and size distribution determination of aggregated virus populations by asymmetric flow field-flow fractionation and multiangle light scattering techniques

  1. Matt McEvoy†,
  2. Vladimir Razinkov‡,
  3. Ziping Wei*,
  4. Jose R. Casas-Finet,
  5. Guillermo I. Tous§,
  6. Mark A. Schenerman

Article first published online: 7 FEB 2011

DOI: 10.1002/btpr.499

Issue

Biotechnology Progress

Biotechnology Progress

Volume 27, Issue 2, pages 547–554, March/April 2011

Additional Information

How to Cite

McEvoy, M., Razinkov, V., Wei, Z., Casas-Finet, J. R., Tous, G. I. and Schenerman, M. A. (2011), Improved particle counting and size distribution determination of aggregated virus populations by asymmetric flow field-flow fractionation and multiangle light scattering techniques. Biotechnol Progress, 27: 547–554. doi: 10.1002/btpr.499

Author Information

  1. Dept. of Analytical Biochemistry, MedImmune, One MedImmune Way, Gaithersburg, MD 20878

  1. †

    18162 Truffle Lane, Germantown, MD 20841

  2. ‡

    Amgen, Inc., 1201 Amgen Court West, Seattle, WA 98119

  3. §

    STIEFEL, a GSK company, 20 T.W. Alexander Drive, Research Triangle Park, NC 27709

*Dept. of Analytical Biochemistry, MedImmune, One MedImmune Way, Gaithersburg, MD 20878

Publication History

  1. Issue published online: 11 APR 2011
  2. Article first published online: 7 FEB 2011
  3. Accepted manuscript online: 25 AUG 2010 09:25AM EST
  4. Manuscript Revised: 14 JUN 2010
  5. Manuscript Received: 23 FEB 2010

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Keywords:

  • AFFFF;
  • MALS;
  • virus counting;
  • particle counting;
  • particle size;
  • AFM

Abstract

A method using a combination of asymmetric flow field-flow fractionation (AFFFF) and multiangle light scattering (MALS) techniques has been shown to improve the estimation of virus particle counts and the amount of aggregated virus in laboratory samples. The method is based on the spherical particle counting approach given by Wyatt and Weida in 2004, with additional modifications. The new method was tested by analyzing polystyrene beads and adenovirus samples, both having a well-characterized particle size and concentration. Influenza virus samples were analyzed by the new AFFFF-MALS technique, and particle size and aggregate state were compared with results from atomic force microscopy analysis. The limitations and source of possible errors for the new AFFFF-MALS analysis are discussed. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010

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