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Applied Cellular Physiology and Metabolic Engineering
Efficient production of D-glucosaminic acid from D-glucosamine by Pseudomonas putida GNA5
Article first published online: 23 DEC 2010
DOI: 10.1002/btpr.529
Copyright © 2010 American Institute of Chemical Engineers (AIChE)
Additional Information
How to Cite
Wu, B., Bai, Z., Meng, X. and He, B. (2011), Efficient production of D-glucosaminic acid from D-glucosamine by Pseudomonas putida GNA5. Biotechnol Progress, 27: 32–37. doi: 10.1002/btpr.529
Publication History
- Issue published online: 10 FEB 2011
- Article first published online: 23 DEC 2010
- Accepted manuscript online: 8 NOV 2010 08:43AM EST
- Manuscript Revised: 12 SEP 2010
- Manuscript Received: 23 APR 2010
Funded by
- National Program on Key Basic Research Project. Grant Number: 2009CB724700
- National High-tech R&D Program of China. Grant Number: 2007AA021306
- National Key Technology R&D Program. Grant Number: 2008BAI63B07
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Keywords:
- D-glucosaminic acid;
- Pseudomonas putida;
- D-glucosamine;
- oxidative fermentation
Abstract
D-glucosaminic acid was produced efficiently from glucosamine by oxidative fermentation using a newly isolated strain, Pseudomonas putida GNA5. After optimization of the fermentation process, 51.5 g L−1D-glucosaminic acid was produced from an initial concentration of 60 g L−1D-glucosamine-HCl after 72 h of oxidative fermentation, which corresponded to a molar yield of 95.4%. This production process is potentially of considerable economic significance because very few by-products were detected. Furthermore, D-glucosaminic acid was accumulated stably during the oxidative fermentation process without the addition of an inhibitor of D-glucosaminic acid breakdown, even though D-glucosamine was exhausted. These results suggest that the mechanisms of D-glucosaminic acid-related metabolism differ between Pseudomonas putida GNA5 and the strain Pseudomonas genera, which was previously reported to produce D-glucosaminic acid. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2011