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Cell Culture and Tissue Engineering
High-density microcarrier cell cultures for influenza virus production
Article first published online: 7 JAN 2011
DOI: 10.1002/btpr.539
Copyright © 2011 American Institute of Chemical Engineers (AIChE)
Additional Information
How to Cite
Bock, A., Schulze-Horsel, J., Schwarzer, J., Rapp, E., Genzel, Y. and Reichl, U. (2011), High-density microcarrier cell cultures for influenza virus production. Biotechnol Progress, 27: 241–250. doi: 10.1002/btpr.539
Publication History
- Issue published online: 10 FEB 2011
- Article first published online: 7 JAN 2011
- Accepted manuscript online: 12 NOV 2010 10:53AM EST
- Manuscript Revised: 1 NOV 2010
- Manuscript Received: 31 MAY 2010
- Abstract
- Article
- References
- Cited By
Keywords:
- high-density cell culture;
- MDCK;
- microcarrier;
- influenza;
- vaccine
Abstract
Influenza virus A/PR/8/34 virus propagation in adherent Madin–Darby canine kidney cells in high-density microcarrier cultures is described. To improve virus yields, perfusion and repeated fed-batch modes were applied using cell-specific feed rates. Cell densities up to 1.1 × 107 cells/mL were achieved. Cell-specific virus yields in high-density cultures were at similar levels compared with standard, low-density cultivations. In the average 2,400 and 3,300 virions per cell were obtained for two variants of the virus strain A/PR/8/34, PR8-National Institute for Biological Standards and Control (NIBSC) and PR8-Robert Koch Institute, respectively. Maximum virus titer (HA activity = 1,778 HAU/100 μL) for virus variant PR8-NIBSC was obtained for a cultivation infected before maximum cell concentration was reached. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011