Applied Cellular Physiology and Metabolic Engineering

Screening 64 cultivars Catharanthus roseus for the production of vindoline, catharanthine, and serpentine

  1. Ill-Min Chung1,
  2. Eun-Hye Kim1,
  3. Mai Li2,
  4. Christie A. M. Peebles2,
  5. Woo-Suk Jung1,
  6. Hog-Keun Song1,
  7. Joung-Kuk Ahn1,
  8. Ka-Yiu San2,*

Article first published online: 14 JUN 2011

DOI: 10.1002/btpr.557

Issue

Biotechnology Progress

Biotechnology Progress

Volume 27, Issue 4, pages 937–943, July/August 2011

Additional Information

How to Cite

Chung, I.-M., Kim, E.-H., Li, M., Peebles, C. A. M., Jung, W.-S., Song, H.-K., Ahn, J.-K. and San, K.-Y. (2011), Screening 64 cultivars Catharanthus roseus for the production of vindoline, catharanthine, and serpentine. Biotechnol Progress, 27: 937–943. doi: 10.1002/btpr.557

Author Information

  1. 1

    Dept. of Applied Life Science, Konkuk University, Seoul 143-701, Korea

  2. 2

    Dept. of Bioengineering, Rice University, Houston, TX 77005

*Dept. of Bioengineering, Rice University, Houston, TX 77005

Publication History

  1. Issue published online: 3 AUG 2011
  2. Article first published online: 14 JUN 2011
  3. Accepted manuscript online: 15 DEC 2010 09:55AM EST
  4. Manuscript Revised: 28 OCT 2010
  5. Manuscript Received: 9 MAY 2010

Funded by

  • Konkuk University

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Keywords:

  • reversed phase HPLC;
  • catharanthine;
  • vindoline;
  • serpentine;
  • C. roseus germplasm;
  • terpenoid indole alkaloids

Abstract

The leaves of Catharanthus roseus (L.) G. Don. are a valuable source of the terpenoid indole alkaloid (TIA) anticancer drugs, vinblastine and vincristine. In particular, the precursor molecules vindoline and catharanthine are harvested from leaves and used for the semisynthetic production of vinblastine and vincristine. Because of this application, catharanthine and vindoline can be used to screen for high-yielding TIA cultivars. In this study, we compared the TIA concentrations in the leaves of 64 different cultivars of C. roseus in the soil experiments. The highest concentration of serpentine was found in Cooler Rose Hot (461 ± 46 μg/g DW). Concentrations of vindoline (2082 ± 113 μg/g DW) and catharanthine (2903 ± 384 μg/g DW) were highest in Pacifica Peach. To eliminate the abiotic and biotic effects of the soils on the plant growth, sterile agar experiments were performed to investigate the TIA concentrations and mRNA transcript levels of selected TIA pathway genes. Six cultivars were investigated (two each of the high level, mid level, and low level producers of TIAs). © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011

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