Applied Cellular Physiology and Metabolic Engineering

A model-based method for investigating bioenergetic processes in autotrophically growing eukaryotic microalgae: Application to the green algae Chlamydomonas reinhardtii

  1. Guillaume Cogne1,*,
  2. Marco Rügen2,
  3. Alexander Bockmayr2,
  4. Mariana Titica1,
  5. Claude-Gilles Dussap3,
  6. Jean-François Cornet4,
  7. Jack Legrand1

Article first published online: 12 MAY 2011

DOI: 10.1002/btpr.596

Issue

Biotechnology Progress

Biotechnology Progress

Volume 27, Issue 3, pages 631–640, May/June 2011

Additional Information

How to Cite

Cogne, G., Rügen, M., Bockmayr, A., Titica, M., Dussap, C.-G., Cornet, J.-F. and Legrand, J. (2011), A model-based method for investigating bioenergetic processes in autotrophically growing eukaryotic microalgae: Application to the green algae Chlamydomonas reinhardtii. Biotechnol Progress, 27: 631–640. doi: 10.1002/btpr.596

Author Information

  1. 1

    GEPEA - UMR CNRS 6144, Université de Nantes, Bât. CRTT, 37 bd de l'Université, BP 406, F-44602 Saint-Nazaire Cedex, France

  2. 2

    DFG Research Center Matheon, Freie Universität Berlin, Arnimallee 3, D-14195 Berlin, Germany

  3. 3

    Clermont Université, Université Blaise Pascal, EA 3866 - Laboratoire de Génie Chimique et Biochimique, BP 10448, F-63000 Clermont-Ferrand Cedex, France

  4. 4

    Clermont Université, ENSCCF, EA 3866 - Laboratoire de Génie Chimique et Biochimique, BP 10448, F-63000 Clermont-Ferrand Cedex, France

*GEPEA - UMR CNRS 6144, Université de Nantes, Bât. CRTT, 37 bd de l'Université, BP 406, F-44602 Saint-Nazaire Cedex, France

Publication History

  1. Issue published online: 6 JUN 2011
  2. Article first published online: 12 MAY 2011
  3. Accepted manuscript online: 22 MAR 2011 03:27PM EST
  4. Manuscript Revised: 6 JAN 2011
  5. Manuscript Received: 2 NOV 2010

Funded by

  • French ANR Project “ALGOMICS”. Grant Number: ANR-08-BIOE-002
  • FP7 European Project “SOLAR-H2”

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Keywords:

  • metabolic modeling;
  • eukaryotic microalgae;
  • thermodynamic constraints;
  • mixed integer programming

Abstract

A constraint-based modeling approach was developed to investigate the metabolic response of the eukaryotic microalgae Chlamydomonas reinhardtii under photoautotrophic conditions. The model explicitly includes thermodynamic and energetic constraints on the functioning metabolism. A mixed integer linear programming method was used to determine the optimal flux distributions with regard to this set of constraints. It enabled us, in particular, to highlight the existence of a light-driven respiration depending on the incident photon flux density in photobioreactors functioning in physical light limitation. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011

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