Process Sensing and Control

In vitro cell culture pO2 is significantly different from incubator pO2

  1. L. L. Bambrick1,
  2. Y. Kostov2,†,
  3. G. Rao2,†,*

Article first published online: 26 MAY 2011

DOI: 10.1002/btpr.622

Issue

Biotechnology Progress

Biotechnology Progress

Volume 27, Issue 4, pages 1185–1189, July/August 2011

Additional Information

How to Cite

Bambrick, L. L., Kostov, Y. and Rao, G. (2011), In vitro cell culture pO2 is significantly different from incubator pO2. Biotechnol Progress, 27: 1185–1189. doi: 10.1002/btpr.622

Author Information

  1. 1

    Dept. of Anesthesiology, University of Maryland School of Medicine, Baltimore. MD 21201

  2. 2

    Center for Advanced Sensor Technology, Dept. of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, MD 21250

  1. †

    Conflicts of interest: G.R. and Y.K. have an equity position in Fluorometrix, Corporation

*Dept. of Chemical and Biochemical Engineering, Center for Advanced Sensor Technology, University of Maryland Baltimore County, Baltimore, MD 21250

  1. †

    Conflicts of interest: G.R. and Y.K. have an equity position in Fluorometrix, Corporation

Publication History

  1. Issue published online: 3 AUG 2011
  2. Article first published online: 26 MAY 2011
  3. Accepted manuscript online: 20 APR 2011 12:38PM EST
  4. Manuscript Revised: 21 DEC 2010
  5. Manuscript Received: 4 NOV 2010

Funded by

  • Sartorius-Stedim Biotechnology

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Keywords:

  • oxygen;
  • neurons;
  • astrocytes;
  • sensor;
  • adherent cells;
  • cell culture

Abstract

Continuous noninvasive monitoring of peri-cellular liquid phase pO2 in adherent cultures is described. For neurons and astrocytes, this approach demonstrates that there is a significant difference between predicted and observed liquid phase pO2. Particularly at low gas phase pO2s, cell metabolism shifts liquid phase pO2 significantly lower than would be predicted from the O2 gas/air equilibrium coefficient, indicating that the cellular oxygen uptake rate exceeds the oxygen diffusion rate. The results demonstrate the need for direct pO2 measurements at the peri-cellular level, and question the widely adopted current practice of relying on setting the incubator gas phase level as means of controlling pericellular oxygen tension, particularly in static culture systems that are oxygen mass transfer limited. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011

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