Cell Culture and Tissue Engineering

Microfibrous carriers for cell culture: A comparative study

  1. Yuan Wen†,
  2. Shang-Tian Yang*

Article first published online: 23 MAY 2011

DOI: 10.1002/btpr.625

Issue

Biotechnology Progress

Biotechnology Progress

Volume 27, Issue 4, pages 1126–1136, July/August 2011

Additional Information

How to Cite

Wen, Y. and Yang, S.-T. (2011), Microfibrous carriers for cell culture: A comparative study. Biotechnol Progress, 27: 1126–1136. doi: 10.1002/btpr.625

Author Information

  1. William G. Lowrie Dept. of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210

  1. †

    Life Technologies Corporation, 3175 Staley Road, Grand Island, NY 14072

*Dept. of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210

Publication History

  1. Issue published online: 3 AUG 2011
  2. Article first published online: 23 MAY 2011
  3. Accepted manuscript online: 20 APR 2011 09:59AM EST
  4. Manuscript Revised: 16 MAR 2011
  5. Manuscript Received: 30 NOV 2010

Funded by

  • NSF Center for Affordable Nanoengineering of Polymeric Biomedical Devices. Grant Number: EEC-0425626

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Keywords:

  • microcarrier culture;
  • fibrous carrier;
  • CHO;
  • cell adsorption;
  • adhesion;
  • polyethylene terephthalate

Abstract

Small patches of polyethylene terephthalate (PET) nonwoven microfibrous matrices have excellent properties and can be used as carriers for culturing cells in agitated bioreactors. The microfibrous carriers are highly porous and can provide large surface areas and three-dimensional space for high-density cell growth. In this work, the microfibrous carriers and several commercial microcarriers were used to study cell attachment kinetics, growth, and monoclonal antibody production with Chinese hamster ovary cells. Compared with commercial solid and macroporous microcarriers, the microfibrous carriers showed better or similar performances. In addition, the microfibrous carriers provided a wider operable range for agitation rate than commercial microcarriers, effectively protecting cells from shear stress and carrier collisions. In addition, the microfibrous carriers are available at a much lower cost than commercial microcarriers, providing an attractive alternative to microcarrier-based large-scale cell cultures. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011

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