Formulation and Engineering of Biomaterials

Fermentation medium for collagenase production by Penicillium aurantiogriseum URM4622

  1. Carolina A. Lima1,
  2. Daniela A. Viana Marques2,
  3. Benício Barros Neto3,
  4. José L. Lima Filho4,5,
  5. Maria G. Carneiro-da-Cunha4,5,
  6. Ana L. F. Porto6,7,*

Article first published online: 19 JUL 2011

DOI: 10.1002/btpr.664

Issue

Biotechnology Progress

Biotechnology Progress

Volume 27, Issue 5, pages 1470–1477, September/October 2011

Additional Information

How to Cite

Lima, C. A., Viana Marques, D. A., Neto, B. B., Lima Filho, J. L., Carneiro-da-Cunha, M. G. and Porto, A. L. F. (2011), Fermentation medium for collagenase production by Penicillium aurantiogriseum URM4622. Biotechnol Progress, 27: 1470–1477. doi: 10.1002/btpr.664

Author Information

  1. 1

    Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Av. Prof. Moraes Lins do Rego, s/n, 50670-901, Recife, PE, Brazil

  2. 2

    Dept. of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 16, Cidade Universitária, CEP 05508-000, São Paulo, SP, Brazil

  3. 3

    Dept. of Fundamental Chemistry, Federal University of Pernambuco, Av. Prof. Luís Freire, s/n, CEP 50740-540, Recife, Pernambuco, Brazil

  4. 4

    Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Av. Prof. Moraes Lins do Rego, s/n, 50670-901, Recife, PE, Brazil

  5. 5

    Dept. of Biochemistry, Federal University of Pernambuco, Av. Professor Moraes Rego, s/n, Campus Universitário 50670-901, Recife, PE, Brazil

  6. 6

    Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Av. Prof. Moraes Lins do Rego, s/n, 50670-901, Recife, PE, Brazil

  7. 7

    Dept. of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, PE, Brazil

*Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Av. Prof. Moraes Lins do Rego, s/n, 50670-901, Recife, PE, Brazil

Publication History

  1. Issue published online: 10 OCT 2011
  2. Article first published online: 19 JUL 2011
  3. Accepted manuscript online: 17 JUN 2011 07:17AM EST
  4. Manuscript Revised: 17 MAY 2011
  5. Manuscript Received: 28 FEB 2011

Funded by

  • CNPq

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Keywords:

  • collagenase;
  • optimization;
  • inexpensive media;
  • central composite design;
  • Penicillium aurantiogriseum

Abstract

Medium composition and culture conditions for maximal collagenase production by Penicillium aurantiogriseum URM4622 were optimized using a response surface approach. A full two-level design on three factors (initial medium pH, soybean flour concentration, and temperature) was employed to identify the most significant fermentation parameters for collagenase production, and a subsequent central composite design (CCD) was used to find the optimal levels of the two most significant factors (initial medium pH and soybean flour concentration). The design results indicated that the initial medium pH and the temperature had significant negative main effects, whereas the substrate concentration had a positive effect on the collagenase production. The maximum collagenolytic activity predicted by the fitted response surface was expected to occur at pH 7.21, 1.645% soybean flour concentration and 24°C. Three replicate experiments were run at these conditions and yielded an activity response of 283.36 ± 1.33 U, which not only is the highest obtained in this study but also represents a 5-fold increase over the lowest response observed in the initial design. Since all experiments were carried out with an inexpensive substrate, the final results point out to a cost-effective medium for collagenase production with potential industrial-scale applications. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011

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