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Cell Culture and Tissue Engineering
Computational fluid dynamics modeling of mass transfer behavior in a bioreactor for hairy root culture. I. Model development and experimental validation
Article first published online: 23 AUG 2011
DOI: 10.1002/btpr.682
Copyright © 2011 American Institute of Chemical Engineers (AIChE)
Additional Information
How to Cite
Liu, R., Sun, W. and Liu, C.-Z. (2011), Computational fluid dynamics modeling of mass transfer behavior in a bioreactor for hairy root culture. I. Model development and experimental validation. Biotechnol Progress, 27: 1661–1671. doi: 10.1002/btpr.682
Publication History
- Issue published online: 1 DEC 2011
- Article first published online: 23 AUG 2011
- Manuscript Revised: 24 JUN 2011
- Manuscript Received: 28 MAR 2011
Funded by
- National Basic Research Program. Grant Number: 973 Program
- National Basic Research Program China. Grant Number: 2011CB200903
- Chinese Academy of Sciences. Grant Number: YZ200947, YZ-06-03, KSCX2-YW-R-2, and KSCX1-YW-11D1
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- Cited By
Keywords:
- computational fluid dynamics;
- multiphase flow;
- hydrodynamics;
- mass transfer;
- hairy root
Abstract
A two-dimensional axisymmetric computational fluid dynamics (CFD) model based on a porous media model and a discrete population balance model was established to investigate the hydrodynamics and mass transfer behavior in an airlift bioreactor for hairy root culture. During the hairy root culture of Echinacea purpurea, liquid and gas velocity, gas holdup, mass transfer rate, as well as oxygen concentration distribution in the airlift bioreactor were simulated by this CFD model. Simulative results indicated that liquid flow and turbulence played a dominant role in oxygen mass transfer in the growth domain of the hairy root culture. The dissolved oxygen concentration in the hairy root clump increased from the bottom to the top of the bioreactor cultured with the hairy roots, which was verified by the experimental detection of dissolved oxygen concentration in the hairy root clump. This methodology provided insight understanding on the complex system of hairy root culture and will help to eventually guide the bioreactor design and process intensification of large-scale hairy root culture. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011