Process Sensing and Control

The potential of spatially resolved spectroscopy for monitoring angiogenesis in the chorioallantoic membrane

  1. Eva Verhoelst1,*,
  2. Flip Bamelis1,
  3. Bart De Ketelaere1,
  4. Nghia Nguyen Do Trong1,
  5. Josse De Baerdemaeker1,
  6. Wouter Saeys1,
  7. Mizuki Tsuta2,
  8. Eddy Decuypere3

Article first published online: 20 SEP 2011

DOI: 10.1002/btpr.697

Issue

Biotechnology Progress

Biotechnology Progress

Volume 27, Issue 6, pages 1785–1792, November/December 2011

Additional Information

How to Cite

Verhoelst, E., Bamelis, F., Ketelaere, B. D., Trong, N. N. D., Baerdemaeker, J. D., Saeys, W., Tsuta, M. and Decuypere, E. (2011), The potential of spatially resolved spectroscopy for monitoring angiogenesis in the chorioallantoic membrane. Biotechnol Progress, 27: 1785–1792. doi: 10.1002/btpr.697

Author Information

  1. 1

    Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium

  2. 2

    National Food Research Institute, 2-1-12 Kan-nondai, Tsukuba, Ibaraki 305-8642, Japan

  3. 3

    Faculty of Bioscience Engineering, Dept. of Biosystems, Division of Livestock-Nutrition-Quality, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium

*Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium

Publication History

  1. Issue published online: 1 DEC 2011
  2. Article first published online: 20 SEP 2011
  3. Manuscript Revised: 3 MAY 2011
  4. Manuscript Received: 8 FEB 2011

Funded by

  • Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
  • Industrial Research Fund (Industrieel Onderzoeksfonds, IOF)
  • Research Foundation—Flanders (FWO)
  • Japanese Society for the Promotion of Science (JSPS)

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Keywords:

  • spatially resolved spectroscopy;
  • chorioallantoic membrane;
  • angiogenesis;
  • chicken embryo

Abstract

Over the last decade, the poultry sector has sought to develop ways to monitor chicken embryonic development as to optimize the incubation conditions. One of the parameters of development which may change under different incubation conditions is the angiogenesis in the chorioallantoic membrane (CAM). To be able to quantify these changes in the angiogenesis and detect long-term effects on health, a non-destructive technique is necessary. In this article, the first steps toward such a non-destructive technique are successfully taken. A spatially resolved spectroscopy set-up is built and tested for its potential to measure changes in angiogenesis with incubation time, and differences between a normal and hypercapnic incubation. In this first study, reflectance measurements are performed directly on the CAM as the eggshell considerably complicates the analysis. This issue should be addressed in future research to come to a really non-destructive technique. An experiment was conducted in which one group was incubated under normal conditions, and another under early prenatal hypercapnic conditions (i.e., increased CO2 concentrations). The angiogenesis in the CAM was measured at embryonic day (ED) 10, 13, and 16. The measurements showed a clear blood spectrum with an increasing amount of blood in time, and significant differences in the reflectance as function of the source-detector distances. However, no significant differences between the hypercapnia and the control group could be detected. © 2011 American Institute of Chemical Engineers Biotechnol. Prog.,, 2011

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