The potential of spatially resolved spectroscopy for monitoring angiogenesis in the chorioallantoic membrane

Authors

  • Eva Verhoelst,

    Corresponding author
    1. Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium
    • Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium
    Search for more papers by this author
  • Flip Bamelis,

    1. Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium
    Search for more papers by this author
  • Bart De Ketelaere,

    1. Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium
    Search for more papers by this author
  • Nghia Nguyen Do Trong,

    1. Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium
    Search for more papers by this author
  • Josse De Baerdemaeker,

    1. Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium
    Search for more papers by this author
  • Wouter Saeys,

    1. Faculty of Bioscience Engineering, Dept. of Biosystems, Division of MeBioS, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium
    Search for more papers by this author
  • Mizuki Tsuta,

    1. National Food Research Institute, 2-1-12 Kan-nondai, Tsukuba, Ibaraki 305-8642, Japan
    Search for more papers by this author
  • Eddy Decuypere

    1. Faculty of Bioscience Engineering, Dept. of Biosystems, Division of Livestock-Nutrition-Quality, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium
    Search for more papers by this author

Abstract

Over the last decade, the poultry sector has sought to develop ways to monitor chicken embryonic development as to optimize the incubation conditions. One of the parameters of development which may change under different incubation conditions is the angiogenesis in the chorioallantoic membrane (CAM). To be able to quantify these changes in the angiogenesis and detect long-term effects on health, a non-destructive technique is necessary. In this article, the first steps toward such a non-destructive technique are successfully taken. A spatially resolved spectroscopy set-up is built and tested for its potential to measure changes in angiogenesis with incubation time, and differences between a normal and hypercapnic incubation. In this first study, reflectance measurements are performed directly on the CAM as the eggshell considerably complicates the analysis. This issue should be addressed in future research to come to a really non-destructive technique. An experiment was conducted in which one group was incubated under normal conditions, and another under early prenatal hypercapnic conditions (i.e., increased CO2 concentrations). The angiogenesis in the CAM was measured at embryonic day (ED) 10, 13, and 16. The measurements showed a clear blood spectrum with an increasing amount of blood in time, and significant differences in the reflectance as function of the source-detector distances. However, no significant differences between the hypercapnia and the control group could be detected. © 2011 American Institute of Chemical Engineers Biotechnol. Prog.,, 2011

Ancillary