Formulation and Engineering of Biomaterials

Enterovirus 71 adsorption on metal ion-composite chitosan beads

  1. Ya-Ching Lin*,
  2. Shu-Ting Lin,
  3. Cheng-Yi Chen,
  4. Sheng-Chi Wu

Article first published online: 20 SEP 2011

DOI: 10.1002/btpr.699

Issue

Biotechnology Progress

Biotechnology Progress

Volume 28, Issue 1, pages 206–214, January/February 2012

Additional Information

How to Cite

Lin, Y.-C., Lin, S.-T., Chen, C.-Y. and Wu, S.-C. (2012), Enterovirus 71 adsorption on metal ion-composite chitosan beads. Biotechnol Progress, 28: 206–214. doi: 10.1002/btpr.699

Author Information

  1. Dept. of Biotechnology, Fooyin University, Kaohsiung City, Taiwan, P.R. China

*Dept. of Biotechnology, Fooyin University, Kaohsiung City, Taiwan, P.R. China

Publication History

  1. Issue published online: 2 FEB 2012
  2. Article first published online: 20 SEP 2011
  3. Accepted manuscript online: 4 AUG 2011 10:57AM EST
  4. Manuscript Revised: 13 JUL 2011
  5. Manuscript Received: 20 FEB 2011

Funded by

  • National Science Council (NSC). Grant Number: NSC 97-2221-E-242-002
Corrected by:

Erratum: Erratum

Vol. 28, Issue 3, 892, Article first published online: 20 MAY 2012

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Keywords:

  • enterovirus 71;
  • chitosan;
  • adsorption;
  • metal ions

Abstract

In this study, we developed composite chitosan beads combining various metal ions, including Ni2+, Cu2+, Zn2+, and Fe2+, for direct adsorption of enterovirus 71 (EV71). The metal-ion species had significant effects on the adsorption capacity of beads. Among these metal ion-composite chitosan beads, Ni2+-chitosan beads exhibited the best adsorption capacity of EV71. Using a concentration of 0.01-M Ni2+ was found to best provide for bead formation and EV71 adsorption. The adsorption of EV71 for Ni2+-chitosan beads at neutral or alkaline pH was favored. Under a competitive condition with albumin proteins, Ni2+-chitosan beads exhibited significant capacity of EV71 adsorption in culture media. The adsorption of EV71 on the Ni2+-chitosan beads was attributed to the strong binding between Ni2+ ions chelated to the surface amino acid of EV71 capsids and Ni2+ ions chelated on the chitosan materials. Moreover, the adsorbed EV71 retained its antigenicity and infectivity after desorption. The Ni2+-chitosan beads exhibit a promising application to EV71 adsorption and removal. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012

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