Formulation and Engineering of Biomaterials

Nutrient improvement using statistical optimization for growth of Schizophyllum commune, and its antifungal activity against wood degrading fungi of rubberwood

  1. Yi Peng Teoh1,
  2. Mashitah Mat Don1,*,
  3. Salmiah Ujang2

Article first published online: 11 OCT 2011

DOI: 10.1002/btpr.714

Issue

Biotechnology Progress

Biotechnology Progress

Volume 28, Issue 1, pages 232–241, January/February 2012

Additional Information

How to Cite

Teoh, Y. P., Don, M. M. and Ujang, S. (2012), Nutrient improvement using statistical optimization for growth of Schizophyllum commune, and its antifungal activity against wood degrading fungi of rubberwood. Biotechnol Progress, 28: 232–241. doi: 10.1002/btpr.714

Author Information

  1. 1

    School of Chemical Engineering, Universiti Sains Malaysia, 14300 Nibong Tebal, Seberang Perai South, Penang, Malaysia

  2. 2

    Forest Product Div., Chemistry and Wood Protection Programme, Forest Research Institute of Malaysia (FRIM), 52109 Kepong, Selangor Darul Ehsan, Malaysia

*School of Chemical Engineering, Universiti Sains Malaysia, 14300 Nibong Tebal, Seberang Perai South, Penang, Malaysia

Publication History

  1. Issue published online: 2 FEB 2012
  2. Article first published online: 11 OCT 2011
  3. Accepted manuscript online: 6 SEP 2011 08:31AM EST
  4. Manuscript Revised: 27 AUG 2011
  5. Manuscript Received: 26 MAY 2011

Funded by

  • National Science Fellowship (NSF)
  • Ministry of Science, Technology and Innovation (MOSTI) of Malaysia and the Research University (RU). Grant Number: 814057

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Keywords:

  • Schizophyllum commune;
  • wood-degrading fungi;
  • Plackett-Burman design (PBD);
  • Box-Behnken design (BBD);
  • antifungal agent

Abstract

Two statistical tools, Plackett-Burman design (PBD) and Box-Behnken design (BBD) were used to optimize the mycelia growth of Schizophyllum commune with different nutrient components. Results showed that 32.92 g/L of biomass were produced using a medium consisting of 18.74 g/L yeast extract, 38.65 g/L glucose, and 0.59 g/L MgSO4.7H2O. The experimental data fitted well with the model predicted values within 0.09 to 0.77% error. The biomass was also tested for antifungal activity against wood degrading fungi of rubberwood. Results showed that the minimum inhibitory concentration (MIC) values for antifungal activity range from 0.16 to 5.00 μg/μL. The GC-MS analysis indicated that this fungus produced several compounds, such as glycerin, 2(3H)-furanone, 5-heptyldihydro-, 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, and triacetin. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012

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