Note: Cell Culture and Tissue Engineering

Neurite outgrowth of PC12 cells on diX (parylene) family materials

  1. Takayuki Hoshino1,†,
  2. Itsuro Saito2,
  3. Haruka Takano3,
  4. Kazuyuki Samejima4,
  5. Kunihiko Mabuchi5,
  6. Yasuhiro X. Kato4,†,*

Article first published online: 2 NOV 2011

DOI: 10.1002/btpr.739

Issue

Biotechnology Progress

Biotechnology Progress

Volume 28, Issue 2, pages 587–590, March/April 2012

Additional Information

How to Cite

Hoshino, T., Saito, I., Takano, H., Samejima, K., Mabuchi, K. and Kato, Y. X. (2012), Neurite outgrowth of PC12 cells on diX (parylene) family materials. Biotechnol Progress, 28: 587–590. doi: 10.1002/btpr.739

Author Information

  1. 1

    Dept. of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan

  2. 2

    Dept. of Biomedical Engineering, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8656, Japan

  3. 3

    SHIMOJO Implicit Brain Function Project, ERATO, Japan Science and Technology Agency, Kanagawa 243-0198, Japan

  4. 4

    Brain Science Institute, Tamagawa University, Tokyo 194-8610, Japan

  5. 5

    Dept. of Information Physics and Computing, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo 113-8656, Japan

  1. †

    Takayuki Hoshino and Yasuhiro X. Kato contributed equally to this work

*Brain Science Institute, Tamagawa University, Tokyo 194-8610, Japan

  1. †

    Takayuki Hoshino and Yasuhiro X. Kato contributed equally to this work

Publication History

  1. Issue published online: 10 APR 2012
  2. Article first published online: 2 NOV 2011
  3. Accepted manuscript online: 11 OCT 2011 12:31PM EST
  4. Manuscript Revised: 24 SEP 2011
  5. Manuscript Received: 30 JUN 2011

Funded by

  • KAKENHI. Grant Numbers: 23680052, 23700495

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Keywords:

  • diX;
  • parylene;
  • neurite outgrowth;
  • PC12;
  • neural device

Abstract

We investigated neuronal cell differentiation, particularly neurite outgrowth, on the surface of diX H and diX AM using an in vitro examination of a neuron-like rat pheochromocytoma cell line, PC12. diX H and diX AM are in the parylene family of diX C (or Parylene-C), which is widely used as a novel coating material to insulate neural electrodes, and they have been recently commercialized; diX H and diX AM offer different features of biocompatibility. Previously, we found that these new parylene materials have high cell adhesiveness to neuronal cells whereas the adhesiveness of diX C is extremely low. However, their cell differentiation remains unknown although neuronal cell differentiation plays a crucial role in their development and regeneration. This study showed that almost all PC12 cells adhering to the surface of diX AM and diX H were differentiated, but the neurite outgrowth was significantly larger on diX H than that on diX AM and a conventional polystyrene culture dish. The result suggests that diX H may be advantageous as a biocompatible coating material for a scaffold, which can be used on virtually any substrate to get various configurations in neural devices. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012

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