M.T.S. and C.T.V. contributed equally to this work
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Formulation and Engineering of Biomaterials
The incorporation of the A2 protein to produce novel Qβ virus-like particles using cell-free protein synthesis
Article first published online: 28 NOV 2011
DOI: 10.1002/btpr.744
Copyright © 2011 American Institute of Chemical Engineers (AIChE)
Additional Information
How to Cite
Smith, M. T., Varner, C. T., Bush, D. B. and Bundy, B. C. (2012), The incorporation of the A2 protein to produce novel Qβ virus-like particles using cell-free protein synthesis. Biotechnol Progress, 28: 549–555. doi: 10.1002/btpr.744
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M.T.S. and C.T.V. contributed equally to this work
Publication History
- Issue published online: 10 APR 2012
- Article first published online: 28 NOV 2011
- Accepted manuscript online: 28 OCT 2011 03:08PM EST
- Manuscript Revised: 19 OCT 2011
- Manuscript Received: 11 JUL 2011
Funded by
- Office of Research and Creative Activities at Brigham Young University
Abstract
Virus-like particles (VLPs) have been employed for a number of nanometric applications because they self-assemble, exhibit a high degree of symmetry, and can be genetically and chemically modified. However, high symmetry does not allow for a single unique modification site on the VLP. Here, we demonstrate the co-expression of the cytotoxic A2 protein and the coat protein of the bacteriophage Qβ to form a nearly monodispersed population of novel VLPs. Cell-free protein synthesis allows for direct access and optimization of protein-synthesis and VLP-assembly. The A2 is shown to be incorporated at high efficiency, approaching a theoretical maximum of one A2 per VLP. This work demonstrates de novo production of a novel VLP, which contains a unique site that has the potential for future nanometric engineering applications. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012