Characterization of gene localization and accessibility in DHFR-amplified CHO cells

Authors

  • Zhou Jiang,

    1. Dept. of Chemical & Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180
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  • Susan T. Sharfstein

    Corresponding author
    1. Dept. of Chemical & Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180
    • Dept. of Chemical & Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180
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Abstract

Efficient transcription is critical for high yields of recombinant proteins by mammalian cells. We previously reported that dihydrofolate reductase (DHFR)-mediated gene amplification can augment transcriptional rates as well as increasing gene copy numbers in Chinese hamster ovary (CHO) cells.1 In an attempt to elucidate the mechanisms involved, we have employed several approaches to identify the epigenetic differences between cell clones with varying transcriptional rates. Transgene placement and accessibility varies between unrelated parental cell clones with differential transcriptional rates. However, we did not observe any apparent epigenetic differences between parental clones and their amplified progeny, indicating undiscovered regulatory mechanisms are responsible for the augmentation of transcriptional rates upon DHFR-mediated amplification. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009

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