Structure Elucidation and Phytotoxicity of Ecdysteroids from Chenopodium album

Authors

  • Marina DellaGreca,

    1. Dipartimento di Chimica Organica e Biochimica, Università Federico II, Complesso Universitario Monte Sant'Angelo, Via Cinthia 4, I-80126 Napoli (phone: +39-081-674472; fax: +39-081-674393)
    Search for more papers by this author
  • Brigida D'Abrosca,

    1. Dipartimento di Scienze della Vita, II Università di Napoli, Via Vivaldi 43, I-81100 Caserta
    Search for more papers by this author
  • Antonio Fiorentino,

    1. Dipartimento di Scienze della Vita, II Università di Napoli, Via Vivaldi 43, I-81100 Caserta
    Search for more papers by this author
  • Lucio Previtera,

    1. Dipartimento di Chimica Organica e Biochimica, Università Federico II, Complesso Universitario Monte Sant'Angelo, Via Cinthia 4, I-80126 Napoli (phone: +39-081-674472; fax: +39-081-674393)
    Search for more papers by this author
  • Armando Zarrelli

    1. Dipartimento di Chimica Organica e Biochimica, Università Federico II, Complesso Universitario Monte Sant'Angelo, Via Cinthia 4, I-80126 Napoli (phone: +39-081-674472; fax: +39-081-674393)
    Search for more papers by this author

Abstract

The leaves of Chenopodium album were infused in H2O/MeOH. The extract treated with cold acetone gave heavy precipitation, which was removed by centrifugation. Solid material was fractionated into acidic and neutral fractions. The acidic material was subjected to different silica-gel column chromatographies, and then it was purified by reversed-phase HPLC to afford four known ecdysteroids and the new 3β,14α-dihydroxy-5β-pregn-7-ene-2,6,20-trione that were characterized by extensive spectroscopic investigation, especially by 1D- and 2D-NMR. Their effects on germination and growth of Lactuca sativa L. have been studied. The results are reported as percentage differences of germination, root elongation and shoot elongation, from the control at concentrations ranging from 10−4 to 10−7M.

Ancillary