CSIR Emeritus Scientist.
Interaction of Isoquinoline Alkaloids with Polymorphic DNA Structures
Article first published online: 22 SEP 2009
Copyright © 2009 Verlag Helvetica Chimica Acta AG, Zürich
Chemistry & Biodiversity
Volume 6, Issue 9, pages 1323–1342, September 2009
How to Cite
Bhadra, K., Maiti, M. and Kumar, G. (2009), Interaction of Isoquinoline Alkaloids with Polymorphic DNA Structures. Chemistry & Biodiversity, 6: 1323–1342. doi: 10.1002/cbdv.200900017
- Issue published online: 22 SEP 2009
- Article first published online: 22 SEP 2009
- Manuscript Received: 21 JAN 2009
- Isoquinoline alkaloids;
The interaction of berberine, palmatine, and coralyne with the B, Z, and HL form of poly[d(G-C)] was studied. Berberine and palmatine showed moderate binding to the B form, while coralyne showed higher binding, as revealed from spectroscopic and thermodynamic data. Berberine and coralyne binding to the B form was exothermic and enthalpy-driven, while palmatine showed exothermic binding which was favored by both negative enthalpy and negative entropy changes. Berberine and palmatine neither bind nor converted the Z-form structure to B form. Coralyne, on the other hand, exhibited a strong binding affinity to Z DNA structure that was enthalpy-driven. Berberine binding to the HL form was cooperative, exothermic, and favored by both negative enthalpy and negative entropy changes with the formation of an induced CD band. Palmatine showed weak binding, while coralyne showed a strong binding with the HL form. The structural differences in the isoquinoline alkaloids appear to influence the affinity and mode of interactions with these polymorphic DNA structures.