Two Novel Potent α-Amylase Inhibitors from the Family of Acarviostatins Isolated from the Culture of Streptomyces coelicoflavus ZG0656

Authors

  • Peng Geng,

    Corresponding author
    1. Tianjin State Laboratory of Cellular and Molecular Immunology, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, P. R. China, (phone: +86-22-83336816)
    • Tianjin State Laboratory of Cellular and Molecular Immunology, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, P. R. China, (phone: +86-22-83336816)
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  • Ting Sun,

    1. Basic Medical Research Center, Tianjin Medical University, Tianjin 300070, P. R. China
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  • Qiping Zhong,

    1. Tianjin State Laboratory of Cellular and Molecular Immunology, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, P. R. China, (phone: +86-22-83336816)
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  • Xiaoxia Li,

    1. Tianjin State Laboratory of Cellular and Molecular Immunology, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, P. R. China, (phone: +86-22-83336816)
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  • Liying Shi,

    1. Tianjin State Laboratory of Cellular and Molecular Immunology, Department of Microbiology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, P. R. China, (phone: +86-22-83336816)
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  • Fang Bai,

    1. College of Pharmaceutical Sciences, Nankai University, Tianjin 300071, P. R. China
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  • Gang Bai

    1. College of Pharmaceutical Sciences, Nankai University, Tianjin 300071, P. R. China
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Abstract

Two novel aminooligosaccharides were separated from the culture filtrate of Streptomyces coelicoflavus ZG0656. Their chemical structures were determined by acidic hydrolysis, electrospray-ionization tandem mass spectrometry (ESI-MS/MS), and NMR spectroscopy. The compounds were named acarviostatins III0(−1) and III23 according to the nomenclature of this group of metabolites. The two novel acarviostatins were both mixed noncompetitive inhibitors of porcine pancreatic α-amylase (PPA). The inhibition constants (Ki) for acarviostatins III0(−1) and III23 were 0.009 and 0.026 μM, respectively, 151 and 52 times more potent than acarbose.

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