Inhibitory Effects of Maleimide Derivatives from the Mycelia of the Fungus Antrodia cinnamomea BCRC 36799 on Nitric Oxide Production in Lipopolysaccharide (LPS)-Activated RAW264.7 Macrophages

Authors

  • Ming-Der Wu,

    Corresponding author
    1. Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan (phone: +886-3-522-319 (ext 585)
    • Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan (phone: +886-3-522-319 (ext 585)
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    • M.-D. W. and M.-J. C. contributed equally to this work and are co-first authors.

    • Corresponding authors for general information.

    • Corresponding authors for chemical aspects and manuscript.

  • Ming-Jen Cheng,

    Corresponding author
    1. Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan (phone: +886-3-522-319 (ext 585)
    • Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan (phone: +886-3-522-319 (ext 585)
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    • M.-D. W. and M.-J. C. contributed equally to this work and are co-first authors.

    • Corresponding authors for chemical aspects and manuscript.

  • Yi-Jen Yech,

    1. Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan (phone: +886-3-522-319 (ext 585)
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  • Gwo-Fang Yuan,

    Corresponding author
    1. Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan (phone: +886-3-522-319 (ext 585)
    • Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan (phone: +886-3-522-319 (ext 585)
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    • Corresponding authors for general information.

  • Jih-Jung Chen

    1. Graduate Institute of Pharmaceutical Technology, Tajen University (TU), Pingtung 907, Taiwan
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Abstract

Four new maleimide derivatives, antrocinnamomins E–H (14, resp.), together with (3S,4R)-1-hydroxy-3-(4-hydroxyphenyl)-4-(2-methylpropyl)pyrrolidine-2,5-dione (5) and ergosterol were isolated from the mycelia of Antrodia cinnamomea BCRC 36799. The structures were elucidated by 1D- and 2D-NMR spectroscopy, and mass spectrometry. Compounds 15 were evaluated for their inhibitory effects on nitric oxide (NO) production by macrophages. Compounds 2 and 4 showed stronger inhibition of NO production than the positive control quercetin.

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