Research Article

Age-related changes of the endogenous cardiolipin and plasmalogens of guinea pig kidney and their in vitro hydrolysis by endogenous phospholipases: a thin layer chromatographic analysis in conjunction with densitometric measurement

  1. Fatma M. Helmy*,
  2. Marvin H. Hack†,
  3. Amal Juracka

Article first published online: 14 MAY 2003

DOI: 10.1002/cbf.1035

Issue

Cell Biochemistry and Function

Cell Biochemistry and Function

Volume 21, Issue 4, pages 337–344, December 2003

Additional Information

How to Cite

Helmy, F. M., Hack, M. H. and Juracka, A. (2003), Age-related changes of the endogenous cardiolipin and plasmalogens of guinea pig kidney and their in vitro hydrolysis by endogenous phospholipases: a thin layer chromatographic analysis in conjunction with densitometric measurement. Cell Biochem. Funct., 21: 337–344. doi: 10.1002/cbf.1035

Author Information

  1. Department of Biological Sciences, Delaware State University, USA

  1. †

    Deceased.

*Department of Biological Sciences, Delaware State University, 1200 N. Dupont Highway, Dover, DE 19901, USA.

  1. †

    Deceased.

Publication History

  1. Issue published online: 7 NOV 2003
  2. Article first published online: 14 MAY 2003
  3. Manuscript Accepted: 28 NOV 2002
  4. Manuscript Revised: 18 NOV 2002
  5. Manuscript Received: 16 AUG 2002

Funded by

  • National Institute of Health MARC/MORE Division. Grant Number: GM08250-14

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Keywords:

  • cardiolipin;
  • monolysocardiolipin;
  • plasmalogens;
  • lyso alkenyl phosphatidyl ethanolamine;
  • lyso alkenyl phosphatidyl choline;
  • phospholipase A1, A2 (endogenous);
  • guinea pig kidney (pre–post natal)

Abstract

The phosphoglycerides profile of guinea pig kidney, fetal, young adult, and aged, and their in vitro response to the endogenous lipolytic enzymes, mainly in the phospholipase group were determined by TLC technology in conjunction with densitometric measurement. Changes in phosphoglycerides profile subsequent to in vitro incubation of these tissues at pH 7.4, and 38°C for 45 min and prior to phospholipid extraction has provided evidence relating to their respective lipolytic enzymes capabilities and age. These changes are mainly related to endogenous cardiolipin (CL), alkenyl phospholipids (phosphatidyl ethanolamine and phosphatidyl choline) and their endogenous deacylation to their respective lyso derivatives monolysocardiolipin (MLCL), lyso alkenyl phosphatidyl ethanolamine (LPE), and lyso alkenyl phosphatidyl choline (LPC) by endogenous phospholipases. The hydrolysis of the plasmalogen confirms the action of endogenous PLA2 on sn-2 fatty acids of these compounds. Copyright © 2003 John Wiley & Sons, Ltd.

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