Effect of melatonin on cholestatic oxidative stress under constant light exposure
Article first published online: 30 MAY 2003
Copyright © 2003 John Wiley & Sons, Ltd.
Cell Biochemistry and Function
Volume 21, Issue 4, pages 377–380, December 2003
How to Cite
Cruz, A., Padillo, F. J., Granados, J., Túnez, I., Muñoz, M. C., Briceño, J., Pera-Madrazo, C. and Montilla, P. (2003), Effect of melatonin on cholestatic oxidative stress under constant light exposure. Cell Biochem. Funct., 21: 377–380. doi: 10.1002/cbf.1046
- Issue published online: 7 NOV 2003
- Article first published online: 30 MAY 2003
- Manuscript Accepted: 12 DEC 2002
- Manuscript Revised: 26 NOV 2002
- Manuscript Received: 7 OCT 2002
- obstructive jaundice;
- oxidative stress;
This study was designed to evaluate the effect of melatonin on cholestatic oxidative stress under constant light exposure. Cholestasis was induced by double ligature and section of the extra-hepatic bile duct. Melatonin was injected i.p.(1000 μg kg−1 day−1). Malondialdehyde, reduced glutathione, catalase, superoxide dismutase, glutathione reductase, peroxidase and transferase were determined in liver. After bile-duct obstruction and under constant light exposure, an increase in malondialdehyde (p < 0.05) and a slight decrease in reduced glutathione were seen. Enzyme activity, with the exception of glutathione reductase, had significantly diminished. After melatonin administration, malondialdehyde fell (p < 0.001), whereas there was an increase in reduced glutathione (p < 0.0001) compared with untreated controls. Constant light exposure was associated with an increase in hepatic oxidative stress. Treatment with melatonin decreased lipid peroxide synthesis, and permitted a recovery of both reduced glutathione and scavenger enzyme activity. Copyright © 2003 John Wiley & Sons, Ltd.