Neurite outgrowth of NG108-15 cells induced by heat shock protein 90 inhibitors
Article first published online: 17 JUL 2008
Copyright © 2008 John Wiley & Sons, Ltd.
Cell Biochemistry and Function
Volume 26, Issue 8, pages 825–832, December 2008
How to Cite
Jin, E. and Sano, M. (2008), Neurite outgrowth of NG108-15 cells induced by heat shock protein 90 inhibitors. Cell Biochem. Funct., 26: 825–832. doi: 10.1002/cbf.1458
- Issue published online: 27 NOV 2008
- Article first published online: 17 JUL 2008
- Manuscript Accepted: 22 OCT 2007
- Manuscript Revised: 1 OCT 2007
- Manuscript Received: 17 JUL 2007
- Ministry of Education, Science and Culture of Japan. Grant Number: 13680848
- NG108-15 cell;
We previously reported that radicicol (Rad) and geldanamycin (Geld), heat shock protein 90 (Hsp90) inhibitors, potentiate neurite growth of cultured sensory neurons from chick embryo. We now show that the antibiotics induce neurite growth in NG108-15 cells. Treatment of the cells with these drugs caused transient decrease in protein levels of Raf1, ERK1/2, phosphorylated ERK1/2, Akt1, and CDK4. The neurite growth of NG108-15 induced by the inhibitors was blocked by actynomycin D, but the neurite growth stimulated by dbcAMP in the cells was not affected. The neurite growth could be due to a change in the synthesis of some specific protein(s) and is speculated to be due to the transient downregulation of particular-signaling molecules stabilized by Hsp90. Copyright © 2008 John Wiley & Sons, Ltd.