Research Article

You have free access to this content

Adipose-derived stem cell: a better stem cell than BMSC

  1. Yanxia Zhu1,
  2. Tianqing Liu1,*,
  3. Kedong Song1,
  4. Xiubo Fan1,
  5. Xuehu Ma1,
  6. Zhanfeng Cui2

Article first published online: 17 JUL 2008

DOI: 10.1002/cbf.1488

Issue

Cell Biochemistry and Function

Cell Biochemistry and Function

Volume 26, Issue 6, pages 664–675, August 2008

Additional Information

How to Cite

Zhu, Y., Liu, T., Song, K., Fan, X., Ma, X. and Cui, Z. (2008), Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem. Funct., 26: 664–675. doi: 10.1002/cbf.1488

Author Information

  1. 1

    Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, China

  2. 2

    Oxford Centre for Tissue Engineering and Bioprocessing, Department of Engineering Science, Oxford University, Oxford, UK

*Dalian R & D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Liaoning, Dalian 116024, China.

Publication History

  1. Issue published online: 20 AUG 2008
  2. Article first published online: 17 JUL 2008
  3. Manuscript Accepted: 2 APR 2008
  4. Manuscript Revised: 20 FEB 2008
  5. Manuscript Received: 19 AUG 2007

Funded by

  • The National Natural Sciences Foundation of China. Grant Number: 30670525
  • Young Teacher Culture Foundation of Dalian University of Technology. Grant Number: 893228

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (543K)

Keywords:

  • adipose tissue;
  • mesenchymal stem cells;
  • proliferation;
  • doubling time;
  • multipotential

Abstract

To further study the proliferation and multi-differentiation potentials of adipose-derived stem cells (ADSCs), the cells were isolated with improved methods and their growth curves were achieved with cck-8. Surface protein expression was analyzed by flow cytometry to characterize the cell phenotype. The multi-lineage potential of ADSCs was testified by differentiating cells with adipogenic, chondrogenic, osteogenic, and myogenic inducers. The results showed that about 5 × 105 stem cells could be obtained from 400 to 600 mg adipose tissue. The ADSCs can be continuously cultured in vitro for up to 1 month without passage and they have several logarithmic growth phases during the culture period. Also, the flow cytometry analysis showed that ADSCs expressed high levels of stem cell-related antigens (CD13, CD29, CD44, CD105, and CD166), while did not express hematopoiesis-related antigens CD34 and CD45, and human leukocyte antigen HLA-DR was also negative. Moreover, stem cell-related transcription factors, Nanog, Oct-4, Sox-2, and Rex-1 were positively expressed in ADSCs. The expression of alkaline phosphatase (ALP) was detected in the early osteogenic induction and the calcified nodules were observed by von Kossa staining. Intracellular lipid droplets could be observed by Oil Red staining. Differentiated cardiomyocytes were observed by connexin43 fluorescent staining. In order to obtain more stem cells, we can subculture ADSCs every 14 days instead of the normal 5 days. ADSCs still keep strong proliferation ability, maintain their phenotypes, and have stronger multi-differentiation potential after 25 passages. Copyright © 2008 John Wiley & Sons, Ltd.

Get PDF (543K)