Research Article

Ketogenesis evaluation in perfused liver of diabetic rats submitted to short-term insulin-induced hypoglycemia

  1. Helenton Cristhian Barrena1,
  2. Vilma Aparecida Ferreira Godoi Gazola1,
  3. Maria Montserrat Diaz Pedrosa Furlan1,
  4. Rosângela Fernandes Garcia1,
  5. Helenir Medri de Souza2,
  6. Roberto Barbosa Bazotte3,*

Article first published online: 21 JUL 2009

DOI: 10.1002/cbf.1586

Issue

Cell Biochemistry and Function

Cell Biochemistry and Function

Volume 27, Issue 6, pages 383–387, August 2009

Additional Information

How to Cite

Barrena, H. C., Gazola, V. A. F. G., Furlan, M. M. D. P., Garcia, R. F., de Souza, H. M. and Bazotte, R. B. (2009), Ketogenesis evaluation in perfused liver of diabetic rats submitted to short-term insulin-induced hypoglycemia. Cell Biochemistry and Function, 27: 383–387. doi: 10.1002/cbf.1586

Author Information

  1. 1

    Department of Morphophysiological Sciences, State University of Maringá, Maringá, PR, Brazil

  2. 2

    Department of Physiological Sciences, State University of Londrina, Maringá, PR, Brazil

  3. 3

    Department of Pharmacy and Pharmacology, State University of Maringá, Maringá, PR, Brazil

*Department of Pharmacy and Pharmacology, State University of Maringá, 87020-900, Maringá, PR, Brazil.

Publication History

  1. Issue published online: 12 AUG 2009
  2. Article first published online: 21 JUL 2009
  3. Manuscript Accepted: 19 MAY 2009
  4. Manuscript Revised: 29 APR 2009
  5. Manuscript Received: 6 MAR 2009

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (101K)

Keywords:

  • ketogenesis;
  • hypoglycemia;
  • experimental diabetes;
  • insulin;
  • liver metabolism

Abstract

Ketogenesis, inferred by the production of acetoacetate plus ß-hydroxybutyrate, in isolated perfused livers from 24-h fasted diabetic rats submitted to short-term insulin-induced hypoglycemia (IIH) was investigated. For this purpose, alloxan-diabetic rats that received intraperitoneal regular insulin (IIH group) or saline (COG group) injection were compared. An additional group of diabetic rats which received oral glucose (gavage) (100 mg kg−1) 15 min after insulin administration (IIH + glucose group) was included. The studies were performed 30 min after insulin (1.0 U kg−1) or saline injection. The ketogenesis before octanoate infusion was diminished (p < 0.05) in livers from rats which received insulin (COG vs. IIH group) or insulin plus glucose (COG vs. IIH + glucose group). However, the liver ketogenic capacity during the infusion of octanoate (0.3 mM) was maintained (COG vs. IIH group and COG vs. IIH + glucose group). In addition, the blood concentration of ketone bodies was not influenced by the administration of insulin or insulin plus glucose. Taken together, the results showed that inspite the fact that insulin and glucose inhibits ketogenesis, livers from diabetic rats submitted to short-term IIH which received insulin or insulin plus glucose showed maintained capacity to produce acetoacetate and ß-hydroxybutyrate from octanoate. Copyright © 2009 John Wiley & Sons, Ltd.

Get PDF (101K)