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Protective effect of brown Brazilian propolis against acute vaginal lesions caused by herpes simplex virus type 2 in mice: involvement of antioxidant and anti-inflammatory mechanisms

  1. Gláubia Sartori1,
  2. Ana Paula Pesarico1,2,
  3. Simone Pinton1,
  4. Fernando Dobrachinski1,
  5. Silvane Souza Roman1,
  6. Fernanda Pauletto2,
  7. Luiz Carlos Rodrigues Junior2,
  8. Marina Prigol1,*

Article first published online: 24 OCT 2011

DOI: 10.1002/cbf.1810

Issue

Cell Biochemistry and Function

Cell Biochemistry and Function

Volume 30, Issue 1, pages 1–10, January 2012

Additional Information

How to Cite

Sartori, G., Pesarico, A. P., Pinton, S., Dobrachinski, F., Roman, S. S., Pauletto, F., Rodrigues, L. C. and Prigol, M. (2012), Protective effect of brown Brazilian propolis against acute vaginal lesions caused by herpes simplex virus type 2 in mice: involvement of antioxidant and anti-inflammatory mechanisms. Cell Biochem. Funct., 30: 1–10. doi: 10.1002/cbf.1810

Author Information

  1. 1

    Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

  2. 2

    Laboratório de Biologia Molecular e Cultivo de Células, Centro Universitário Franciscano, Santa Maria, RS, Brazil

*Marina Prigol, Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105–900, Santa Maria, RS, Brazil. E-mail: marinaprigol@mail.ufsm.br

Publication History

  1. Issue published online: 3 JAN 2012
  2. Article first published online: 24 OCT 2011
  3. Manuscript Accepted: 24 AUG 2011
  4. Manuscript Revised: 25 JUL 2011
  5. Manuscript Received: 18 MAY 2011

Funded by

  • FAPERGS
  • CAPES
  • CNPq

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Keywords:

  • HSV-2;
  • herpes;
  • propolis;
  • antioxidant;
  • anti-inflammatory

Propolis has been highlighted for its antioxidant, anti-inflammatory and antiviral properties. The purpose of this study was to investigate if brown Brazilian hydroalcoholic propolis extract (HPE) protects against vaginal lesions caused by herpes simplex virus type 2 (HSV-2) in female BALB/c mice. The treatment was divided in 5 days of pre-treatment with HPE [50 mg·kg–1, once a day, intragastric (i.g.)], HSV-2 infection [10 µl of a solution 1 × 102 plaque-forming unit (PFU·ml–1 HSV-2), intravaginal inoculation at day 6] and post-treatment with HPE (50 mg·kg–1) for 5 days more. At day 11, the animals were killed, and the in vivo analysis (score of lesions) and ex vivo analysis [haematological and histological evaluation; superoxide dismutase (SOD), catalase (CAT) and myeloperoxidase (MPO) activities; reactive species (RS), tyrosine nitration levels, non-protein thiols (NPSH) and ascorbic acid (AA) levels] were carried out. HPE treatment reduced extravaginal lesions and the histological damage caused by HSV-2 infection in vaginal tissues of animals. HPE was able to decrease RS, tyrosine nitration, AA levels and MPO activity. Also, it protected against the inhibition of CAT activity in vaginal tissues of mice. HPE promoted protective effect on HSV-2 infected animals by acting on inflammatory and oxidative processes, and this effect probably is caused by its antioxidant and anti-inflammatory properties. Copyright © 2011 John Wiley & Sons, Ltd.

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