Over-expression of circadian clock gene Bmal1 affects proliferation and the canonical Wnt pathway in NIH-3T3 cells
Version of Record online: 7 SEP 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Cell Biochemistry and Function
Volume 31, Issue 2, pages 166–172, March 2013
How to Cite
Lin, F., Chen, Y., Li, X., Zhao, Q. and Tan, Z. (2013), Over-expression of circadian clock gene Bmal1 affects proliferation and the canonical Wnt pathway in NIH-3T3 cells. Cell Biochem. Funct., 31: 166–172. doi: 10.1002/cbf.2871
- Issue online: 5 MAR 2013
- Version of Record online: 7 SEP 2012
- Manuscript Accepted: 8 AUG 2012
- Manuscript Revised: 3 AUG 2012
- Manuscript Received: 2 JUN 2012
- National Natural Science Foundation of China. Grant Number: 30801206
- cell proliferation;
- canonical Wnt pathway;
Bmal1 is a transcription factor that plays a central role in the regulation of circadian rhythms. Recent study reported that Bmal1–/– mice displayed many known features of premature ageing, such as reduction of bone mass. Our previous study has found that both the proliferation of bone marrow mesenchymal stem cells (BMSCs) and Bmal1 expression decreased with advancing age. It seemed that a positive correlation existed between Bmal1 protein level and the proliferative activity of BMSCs. β-catenin, the core factor of the canonical Wnt pathway, also showed reduced expression in aged mice. In order to further confirm this, we constructed a lentiviral vector to over-express Bmal1 in NIH-3T3 cells; successful transfection was verified. The cell proliferation rate of infected cells was higher than the non-transfected NIH-3T3 cells, suggesting that circadian clock gene Bmal1 can promote proliferation. β-catenin showed an increased expression in NIH-3T3 cells after Bmal1 over-expression, indicating that activation of the canonical Wnt pathway might be the mechanism underlying the effect of circadian clock gene Bmal on promoting cell proliferation. Copyright © 2012 John Wiley & Sons, Ltd.