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Protective effect of N-acetylcysteine against ischemia/reperfusion injury in rat urinary bladders

Authors

  • Ju-Hyun Shin,

    1. Department of Urology, School of Medicine, Chungnam National University Hospital, Daejeon, Korea
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    • Authors contributed equally to this work.

  • Gun-Hwa Kim,

    1. Division of Life Science and Pioneer Research Center for Protein Network Exploration, Korea Basic Science Institute (KBSI), Daejeon, Korea
    2. Department of Functional Genomics, University of Science and Technology (UST), Daejeon, Korea
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    • Authors contributed equally to this work.

  • Ki-Hak Song,

    1. Department of Urology, School of Medicine, Chungnam National University Hospital, Daejeon, Korea
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  • Yong-Gil Na,

    1. Department of Urology, School of Medicine, Chungnam National University Hospital, Daejeon, Korea
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  • Chong-Koo Sul,

    1. Department of Urology, School of Medicine, Chungnam National University Hospital, Daejeon, Korea
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  • Jae-Sung Lim

    Corresponding author
    1. Department of Urology, School of Medicine, Chungnam National University Hospital, Daejeon, Korea
    • Correspondence to: Lim Jae-Sung, Department of Urology, School of Medicine Chungnam National University Hospital, Daejeon 301-721, Korea.

      E-mail: uro17@cnuh.co.kr

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Abstract

Ischemia/reperfusion (I/R) injury represents an important cause of bladder contractile dysfunction. One of the major causes leading to this dysfunction is thought to be reactive oxygen species formation. In this study, we investigated the potential benefit of N-acetylcysteine (NAC), a potent antioxidant that neutralizes free radicals, in a rat model of urinary bladder injury. NAC treatment rescues the reduction of contractile response to I/R injury in a dose-dependent manner. In addition, all levels of reactive oxygen species, lipid peroxidation, and NADPH-stimulated superoxide production in the I/R operation + NAC (I/R + NAC) group also decreased compared with a marked increase in the I/R operation + saline (I/R + S) group. Moreover, an in situ fluorohistological approach also showed that NAC reduces the generation of intracellular superoxides enlarged by I/R injury. Together, our findings suggest that NAC has a protective effect against the I/R-induced bladder contractile dysfunction via radical scavenging property. Copyright © 2013 John Wiley & Sons, Ltd.

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