In non-small cell lung cancer mitogenic signaling leaves Sprouty1 protein levels unaffected
Article first published online: 24 APR 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Cell Biochemistry and Function
Volume 32, Issue 1, pages 96–100, January 2014
How to Cite
Kral, R. M., Mayer, C.-E., Vanas, V., Gsur, A. and Sutterlüty-Fall, H. (2014), In non-small cell lung cancer mitogenic signaling leaves Sprouty1 protein levels unaffected. Cell Biochem. Funct., 32: 96–100. doi: 10.1002/cbf.2976
- Issue published online: 8 JAN 2014
- Article first published online: 24 APR 2013
- Manuscript Accepted: 22 MAR 2013
- Manuscript Revised: 4 MAR 2013
- Manuscript Received: 20 DEC 2012
- cell cycle;
Sprouty1 protein belongs to a family of receptor tyrosine kinase-mediated signaling inhibitors, whose members are usually regulated by growth factors to form a negative feedback loop. Correspondingly fluctuations of Sprouty1 mRNA in response to single growth factors have been observed.
In this report, we investigate Sprouty1 protein levels and show that in non-small cell lung carcinoma-derived cells, the expression levels are unaffected by the serum content in the cellular environment. Although cells harboring K-Ras mutations express insignificant higher Sprouty1 levels, ectopic expression of constitutive active Ras in normal human lung fibroblasts fails to augment Sprouty1 protein content. Furthermore, serum starvation for three days has no influence on Sprouty1 expression and addition of serum or of singular growth factors leaves Sprouty protein levels unchanged. Cell cycle analysis reveals that Sprouty1 levels remain constant throughout the whole cell cycle. These data demonstrate that Sprouty1 expression is not connected with mitogenic signaling and cell proliferation. Copyright © 2013 John Wiley & Sons, Ltd.