Walker 256 tumour growth causes marked changes of glutamine metabolism in rat small intestine
Article first published online: 28 NOV 2001
Copyright © 2001 John Wiley & Sons, Ltd.
Cell Biochemistry and Function
Volume 20, Issue 2, pages 107–113, June 2002
How to Cite
Lima, M. M. R., de Mello, M. A. R. and Curi, R. (2002), Walker 256 tumour growth causes marked changes of glutamine metabolism in rat small intestine. Cell Biochem. Funct., 20: 107–113. doi: 10.1002/cbf.961
- Issue published online: 28 NOV 2001
- Article first published online: 28 NOV 2001
- Manuscript Accepted: 30 AUG 2001
- Manuscript Received: 2 JUL 2001
- The British Council
- small intestine;
- Walker 256 tumour;
- phosphate-dependent glutaminase
The effect of Walker 256 tumour growth on the metabolism of glucose and glutamine in the small intestine of rats was examined. Walker 256 tumour has been extensively used as an experimental model to induce cancer cachexia in rats. Walker 256 tumour growth decreased body weight and small intestine weight and length. The activities of glucose-6-phosphate dehydrogenase and phosphate-dependent glutaminase were reduced in the proximal, median and distal portions of the intestine. Glutamine oxidation was reduced in the proximal portion only. The decrease in glutaminase activity was not due to a low synthesis of the protein as indicated by Western blotting analysis. Hexokinase and citrate synthase activities were not changed by the tumour. These findings led us to postulate that tumour growth impairs glutamine metabolism of small intestine but the mechanism involved remains to be elucidated. Copyright © 2001 John Wiley & Sons, Ltd.