On Secondary Structure Rearrangements and Equilibria of Small RNAs



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Any way up: Secondary structure ambivalence is encountered even in very small RNAs with only 20–40 nucleotides (see scheme). A combined synthetic and NMR spectroscopic approach allows simple and reliable verification of small RNA secondary structure equilibria. This approach is discussed in the context of RNA conformational transitions, biologically relevant RNA switches, RNA secondary structure changes induced by natural nucleoside modifications, and a structure and function ambivalent ribozyme sequence.