Short Communication

Probing Protein–Carbohydrate Interactions with Microarrays of Synthetic Oligosaccharides

  1. Daniel M. Ratner1,
  2. Eddie W. Adams1,
  3. Jing Su2,
  4. Barry R. O'Keefe Dr.3,
  5. Milan Mrksich Prof. Dr.2,
  6. Peter H. Seeberger Prof. Dr.1,4

Article first published online: 26 FEB 2004

DOI: 10.1002/cbic.200300804

Issue

ChemBioChem

ChemBioChem

Volume 5, Issue 3, pages 379–383, March 5, 2004

Additional Information

How to Cite

Ratner, D. M., Adams, E. W., Su, J., O'Keefe, B. R., Mrksich, M. and Seeberger, P. H. (2004), Probing Protein–Carbohydrate Interactions with Microarrays of Synthetic Oligosaccharides. ChemBioChem, 5: 379–383. doi: 10.1002/cbic.200300804

Author Information

  1. 1

    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

  2. 2

    Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA

  3. 3

    Molecular Targets Discovery Program, NCI Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA

  4. 4

    Current address: Laboratory for Organic Chemistry, ETH-Hönggerberg HCI F315, Wolfgang-Pauli-Straße 10, 8093 Zürich, Switzerland, Fax: (+41) 1633-1235

Publication History

  1. Issue published online: 26 FEB 2004
  2. Article first published online: 26 FEB 2004
  3. Manuscript Revised: 6 NOV 2003
  4. Manuscript Received: 25 FEB 2003

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Keywords:

  • carbohydrate chips;
  • carbohydrates;
  • lectins;
  • microarrays;
  • proteins

Graphical Abstract

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On the spot: Carbohydrate microarrays, in concert with surface plasmon resonance, are valuable tools in the rapid identification of carbohydrate–protein interactions, like those between high-mannose oligosaccharides and cyanovirin-N (see picture). By utilizing chemical methods amenable to current high-throughput screening technologies, high-density carbohydrate microarrays can be prepared and screened by using conventional and widely accessible DNA microarray screening instrumentation.

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