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The Biosynthesis of Indolocarbazoles in a Heterologous E. coli Host

Authors

  • Chang-Gu Hyun Dr.,

    1. Laboratory for Biosynthetic Chemistry Pharmaceutical Sciences Division, School of Pharmacy University of Wisconsin–Madison 777 Highland Avenue, Madison, WI 53705, USA, Fax: (+1) 608-262-5345
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  • Tsion Bililign,

    1. Laboratory for Biosynthetic Chemistry Pharmaceutical Sciences Division, School of Pharmacy University of Wisconsin–Madison 777 Highland Avenue, Madison, WI 53705, USA, Fax: (+1) 608-262-5345
    2. Joan and Sanford I. Weill Graduate School of Medical Sciences Cornell University New York, NY 10021, USA
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  • Jianchun Liao Dr.,

    1. Laboratory for Biosynthetic Chemistry Pharmaceutical Sciences Division, School of Pharmacy University of Wisconsin–Madison 777 Highland Avenue, Madison, WI 53705, USA, Fax: (+1) 608-262-5345
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  • Jon S. Thorson Prof.

    1. Laboratory for Biosynthetic Chemistry Pharmaceutical Sciences Division, School of Pharmacy University of Wisconsin–Madison 777 Highland Avenue, Madison, WI 53705, USA, Fax: (+1) 608-262-5345
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Abstract

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The antitumor antibiotic rebeccamycin (1), produced by the bacterium Saccharothrix aerocolonigenes, is a prototype of a class of complex natural products called indolocarbazoles. The cloning and characterization of the rebeccamcyin (reb) gene locus from S. aerocolonigenes is reported. The heterologous expression of this intact gene cluster in Escherichia coli led to the production of 1 and two putative biosynthetic intermediates. This work represents the first production of indolocarbazole analogues in the heterologous host E. coli.

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