Elucidation of the Glycosylation Sequence of Mithramycin Biosynthesis: Isolation of 3A-Deolivosylpremithramycin B and Its Conversion to Premithramycin B by Glycosyltransferase MtmGII

Authors

  • Mohammad Nur-e-Alam Dr.,

    1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 725 Rose Street, Lexington, KY 40536-0082, USA, Fax: (+1) 859-257-7564
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  • Carmen Méndez Prof. Dr.,

    1. Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain
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  • José A. Salas Prof. Dr.,

    1. Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain
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  • Jürgen Rohr Prof. Dr.

    1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 725 Rose Street, Lexington, KY 40536-0082, USA, Fax: (+1) 859-257-7564
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Abstract

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The last step at last. The isolation and structure elucidation of the novel premithramycin-type compound 3A-deolivosylpremithramycin B (1) allowed cross-feeding experiments with this compound to two glycosyltransferase-minus mutants of the mithramycin producer S. argillaceus; this has solved the sequence of the last glycosylation steps of mithramycin biosynthesis.

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