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Keywords:

  • BSA-free system;
  • microarrays;
  • protein chips;
  • nanotubes
Thumbnail image of graphical abstract

Sweet home for proteins. Single-walled carbon nanotube (SWNT) film treated with CDI–Tween20 (CT) has been used as a substrate for a BSA-free protein chip. In the absence of BSA, which is conventionally applied as a nonspecific binding inhibitor, protein A, biotinylated BSA, and even small peptide such as 3×FLAG immobilized on the CT-functionalized SWNT film substrates recognize their respective counterparts, streptavidin, immunoglobulin G, and antiFLAG. with high specificity.