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Shotgun Cloning and Heterologous Expression of the Patellamide Gene Cluster as a Strategy to Achieving Sustained Metabolite Production


  • This work was presented at the Society for Industrial Microbiology’s GMBIM/BMP conference held during November 14-18, 2004 at the Hilton San Diego Resort, San Diego, CA.


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Shotgun cloning into E. coli of genomic DNA from Prochloron sp., symbiont of the seasquirt Lissoclinum patella, resulted in the heterologous expression of the patellamide gene cluster and subsequent production of patellamide D (1) and ascidiacyclamide (2) at levels of 80–100 ng mL−1.