These authors contributed equally to this work.
Chloroplastic Glycolipids Fuel Aldehyde Biosynthesis in the Marine Diatom Thalassiosira rotula
Article first published online: 7 FEB 2006
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 7, Issue 3, pages 450–456, March 6, 2006
How to Cite
Cutignano, A., d'Ippolito, G., Romano, G., Lamari, N., Cimino, G., Febbraio, F., Nucci, R. and Fontana, A. (2006), Chloroplastic Glycolipids Fuel Aldehyde Biosynthesis in the Marine Diatom Thalassiosira rotula. ChemBioChem, 7: 450–456. doi: 10.1002/cbic.200500343
- Issue published online: 1 MAR 2006
- Article first published online: 7 FEB 2006
- Manuscript Received: 15 AUG 2005
- chemical ecology;
Enzymatic preparations and specialized analytical tools have shown that chloroplast-derived glycolipids are the main substrates for the biosynthetic pathway that produces antiproliferative polyunsaturated aldehydes in broken cells of the marine diatom Thalassiosira rotula. This process, which is associated with the formation of free fatty acids and lyso compounds from polar lipids but not triglycerides, is largely dependent on glycolipid hydrolytic activity, rather than phospholipase A2 as previously suggested. Preliminary characterization of lipolytic enzymes has revealed protein bands of 40–45 kDa. Under native conditions these proteins seem to be associated with soluble aggregates that have an apparent molecular weight of approximately 200 kDa. The biochemical process, which is similar to that described in the algal-bloom forming diatom Skeletonema costatum, suggests a mechanism based on decompartmentalization and mixing of preexisting enzymes and substrates.